Forschungsbericht 2005

2-09 Bioprozess- und Bioverfahrenstechnik
Bioprozeß- und Bioverfahrenstechnik |
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21073 Hamburg
e-mail: maerkl@tuhh.de
www: http://www.tuhh.de/bt1
Professoren |
|
Prof. Dr.-Ing. Herbert Märkl (Leitung) | (TUHH) |
Sekretariat |
|
Cornelia Hoffmann | (TUHH) |
wissenschaftliche Mitarbeiter/innen |
|
Dipl.-Ing. Michael Buchmann | (TUHH) |
Dipl.-Ing. Burkhard Ernst | (DFG) |
Dipl.-Biol. Christiane Goepfert | (Qualitätsoffensive Hamburg) |
Dipl.-Ing. Philipp Göpel | (BMBF) |
Dipl.-Biol. Matthias Krüger | (High-Tech-Offensive Bayern) |
Dipl.-Ing. Jürgen Kube | (TUHH) |
Priv.-Doz. Dr.-Ing. Ralf Pörtner | (TUHH) |
Dipl.-Ing. Katharina Wiegandt | (Multimediakontor Hamburg) |
sonstige Mitarbeiter/innen |
|
Dipl.-Ing. (FH) Ralf Grajetzki | (TUHH) |
Thomas Gronowski | (TUHH) |
Jan Sens | (TUHH) |
Sigrid Wiebusch | (TUHH) |
Gäste |
|
Prof. Dr. Zakaria Al-Qodah | (DFG) |
Forschungsprojekte
- E.2-09.026 Nachhaltige Produktion hochwertiger Naturfasern
- E.2-09.027 Scale-up eines Verfahrens zur hochdichten Kultivierung rekombinanter Escherichia coli
- E.2-09.029 Novel bioreduction by hyperthermophilic microorganisms for the natural, specific and on-line production of fine chemicals
- E.2-09.032 Ingenieurtechnische Aspekte bei der Herstellung künstlicher Gelenkknorpelimplantate
- E.2-09.034 Interaktive virtuelle Lernumgebung für die Biotechnologie (e-LearnBioTec)
Kooperationen
- Aventis Pharma, Frankfurt/M
- Bioengineering AG, Wald, Schweiz
- Biokraftwerke Fürstenwalde GmbH
- Biomet Merck BioMaterials, Darmstadt
- CellConcept, Umkirch
- Deutsche Hefewerke, Hamburg
- Kurt-Schwabe-Institut, Meinsberg
- Lonza Biologics, Slough, UK
- Prof. Dr. A. Munack, Forschungsanstalt für Landwirtschaft, Braunschweig
- Prof. Dr. M. Al-Rubeai, University of Birmingham, UK
- Prof. Dr. M. Matsumura, University of Tsukuba, Japan
- Prof. Dr. M. Rossi, University of Naples, Neapel, Italien
- Prof. Dr. R. Ladenstein, Karolinska Institute, Stockholm, Schweden
- Prof. Dr. T. Scheper, Universität Hannover
- Prof. Dr. W. de Vos, Wageningen Agricultural University, Wageningen, Niederlande
- Prof. Dr.-Ing. V. Hass, HS Bremen
- Rauschert Verfahrenstechnik GmbH, Steinwiesen
- Universitätsklinikum Eppendorf, Hamburg
- ZellWerk GmbH, Eichstädt
- meredos GmbH, Bovenden
Publikationen
Veröffentlichungen
- 2-09.001V
Müller, R., Antranikian, G., Maloney, S., Sharp, R. (1998) Thermophilic Degradation of Environmental Pollutants. Advances Biochem. Eng../Bio- technol. 61:155-169 - 2-09.002V
Niehaus, F., Frey, B., Antranikian, G. (1997) Cloning and characterisation of a thermostable a-DNA polymerase from the hyperthermophilic archaeon Thermococcus sp. TY. GENE 204:153-158 - 2-09.003V
Mutzel, A., Reinscheid, U. M., Antranikian, G., Müller, R. (1996) Isolation and characterization of thermophilic Bacillus strain, that degrades phenol and cresols as sole carbon source at 70 °C. Appl. Microbiol. Biotechnol. 46: 593-596 - 2-09.005V
Canganella, F., Jones, W. J., Gambacorta, A., Antranikian, G. (1998) Thermococcus guaymasensis sp. nov. and Thermococcus aggregans sp. nov., two novel thermophilic archaea isolated from the Guaymas Basin hydrothermal vent site. Intern. Journal of System. Bacteriol. 48:1181-1185 - 2-09.007V
Märkl, H. ; G. Reinhold: Biogas-Turmreaktor, ein neues Konzept in der anaeroben Abwasserreinigung; Chem. -Ing. Tech. (1994) - 2-09.008V
Friedmann, H. ; H. Märkl,"Der Einfluß von erhöhtem hydrostatischem Druck auf die Biogasproduktion, gwf Nr. 12 (1993) S. 689-698. ". - 2-09.009V
Friedmann, H. ; H. Märkl: Der Einfluß der Produktgase auf die mikrobiologische Methanbildung; gwf (1994) - 2-09.010V
Meyer-Jens, Th. ; Märkl, H. ; Schröder, A. ; Kesners, P. ; Matz, G: Application of a membrane probe for the analysis of mixtures of organic substances and dissolved gaseous components in anaerobic reactors by means of a mass spectrometer. Dechema, Biotechnology conferences (1992), Vol. 5, Part A, S. 391/393 - 2-09.011V
Märkl, H.:Folien und Membranen als neue Elemente im Fermenterbau. forum mikrobiologie 12 (1989), S. 234/237 - 2-09.012V
Märkl, H. , M. Lechner, F. Götz,"A New Dialysis Fermenter for the Production of High Concentrations of Extracellular Enzymes J. Ferment. Bioeng. Vol. 69 (1990), No. 4, S. 244/249. ". - 2-09.013V
Dubach, A. , H. Märkl,"Application of an Extended Kalman Filter Method for Monitoring High Density Cultivation of E. coli. J. Ferment. Bioeng. (1992), Vol. 73, No. 5, S. 296/402. ". - 2-09.014V
Ogbonna, J. Ch; H. Märkl,"Nutrient-split feeding strategy for dialysis cultivation of E. coli. Biotechnology and Bioengineering. (1993), 41, S. 1092/1100. ". - 2-09.015V
Märkl, H. , C. Zenneck, A. Ch. Dubach, J. Ch. Ogbonna: Cultivation of E. coli to high cell densities in a dialysis reactor. Appl. Microbiol. Biotechnol. (1993), 39, S. 48/52. ". - 2-09.016V
Rüdiger, A. , J. Ch. Ogbonna, H. Märkl, G. Antranikian: Effect of gassing, agitation, substrate supplementation and dialysis on the growth of an extremely thermophilic archaeon Pyrococcus woesei. Appl. Microbiol. Biotechnol. (1992), 37, S. 501/504. - 2-09.017V
Wilderer, P. A. ; H. Märkl,"Innovative Reactor Design to Treat Hazardous Wastes. In,"Biotechnology Applications in Hazardous Waste Treatment, Engineering Found. Conf. , Florida, USA, United Engineering Trustees Inc. (1989) S. 214/259. ". - 2-09.018V
Märkl, H. ; C. Zenneck; P. A. Wilderer: Membranbioreaktoren für den mikrobiellen Abbau von BTX-Aromaten; Wasser-Abwasser 132 (1991) Nr. 7. ". - 2-09.019V
Zenneck, C. ; H. Märkl,"Membrane-photo-reactor for microbial degradation of volatile and toxic substanced in,"Arendt, F. ; Annokkee, G. J. ; Bosmann, R. ; van den Brink, W. J. ,"Altlastensanierung '93, 1505-1506, Kluwer Academic Publishers, 1993. ". - 2-09.020V
Kurosawa H. , H. Märkl, C. Niebuhr-Redder; M. Matsumura,"Dialysis Bioreactor with Radial-Flow Fixed Bed for Animal Cell Culture, J. Ferment. Bioeng. , Vol. 72 (1991); No. 1, S. 41/45. ". - 2-09.021V
Bohmann. A. ; R. Pörtner; J. Schmieding; V. Kasche; H. Märkl: Integrated membrane dialysis bioreactor with radial-flow fixed bed - a new approach for continuous cultivation of animal cells,"Cytotechnology, 9,"51-57, 1992". - 2-09.022V
Bohmann, A. ; R. Pörtner; H. Märkl,"Evaluation of optimal process parameters for the membrane dialysis bioreactor with integrated radial fixed bed; In,"Kaminogawa, S. ; Ametani, A. ; Hachimura, S. ; Animal Cell Technology,"Basic & Applied Aspects, Vol. 5, Kluwer Academic Publishers, 1993. ". - 2-09.023V
Lüdemann, I.; R. Pörtner and H. Märkl,"Effect of NH3 on the cell growth of a hybridoma cell line; Cytotechnology 14,"11-20 (1994) ". - 2-09.024V
Pörtner, R.; A. Bohmann; I. Lüdemann and H. Märkl,"Estimation of specific glucose uptake rates in cultures of hybridoma cells,"Journal of Biotechnology 34,"237-246 (1994)". - 2-09.025V
Ong, C.P.; R. Pörtner; H. Märkl; Y. Yamazaki; K. Yasuda and M. Matsumura,"High density cultivation of hybridoma in charged porous carriers; Journal of Biotechnology 34,"259-268 (1994)". - 2-09.026V
Zumbusch, P.v., T. Meyer-Jens, G. Brunner and H. Märkl,"On-line monitoring of organic substances with high-pressure liquid chromatography (HPLC) during anaerobic fermentation of waste-water. Appl. Microbiol. Biotechnol. 42,"140-146 (1994)". - 2-09.027V
Märkl H. und R. Stegmann (Herausgeber),"Anaerobe Behandlung von festen und flüssigen Rückständen. Dechema-Monographie Band 130 (1994)". - 2-09.028V
Meyer-Jens, T.; G. Matz and H. Märkl,"On-line measurement of dissolved and gaseous hydrogen sulphide in anaerobic biogas reactors. Appl. Microbiol. Biotechnol. 43,"341 - 345 (1994)". - 2-09.029V
A. Bohmann; R. Pörtner and H. Märkl,"Performance of a membrane dialysis bioreactor with radial-flow fixed bed for the cultivation of a hybridoma cell line; Appl. Microbiol. Biotechnol. 43,"772-780 (1995) ". - 2-09.030V
O. Holst , A. Manelius, M, Krahe, H. Märkl, N. Raven and R. Sharp,"Thermophiles and Fermentation Technology, submitted to "Comparative Biochemistry and Physiology"". - 2-09.034V
Rüdiger, A., Sunna, A., Antranikian, G.,"Natural polysaccharides degrading enzymes, 316-340. In,"Enzyme Catalysis in Organic Synthesis, Vol. I., K. Drauz and H. Waldmann (eds.) VCH, Weinheim, 1995.". - 2-09.035V
Leuschner, C., Antranikian, G.,"Heat-stable enzymes from extremely thermophilic and hyperthermophilic microorganisms. World journal of Microbiology and Biotechnology, 11, 95-114, 1995.". - 2-09.036V
Rüdiger, A., Jorgensen, P.L., Antranikian, G.,"Isolation and characterization of a heat-stable pullulanase from the hyperthermophilic archaeon Pyrococcus woesei after gene cloning and expression of its gene in Escherichia coli, Appl. Environ. Microbiol., 61, 567-575, 1995.". - 2-09.037V
Sunna, A., Stoffregen, T. and Antranikian, G.,"Xylan degrading enzymes from thermophilic bacteria, pp. 97-99. In Dechema Biotechnology Conferences, Vol. 5A, G. Kreysa and A. J. Driesel (eds.), VCH, Weinheim, Germany, 1992.". - 2-09.038V
Antranikian, G., Rüdiger, A., Canganella, F., Klingeberg, M. and Sunna, A.,"Biodegradation of polymers at temperatures up to 130°C. J. M. S. Pure Appl. Chem. A32,"661-669, 1995.". - 2-09.039V
Sunna, A., Puls, J. and Antranikian, G.,"Characterization of the xylanolytic enzyme system of the extreme thermophilic anaerobic bacteria Thermotoga maritima, T. neapolitana and T. thermarum. Comp. Biochem. Physiol. (im Druck). ". - 2-09.040V
Canganella, F., Andrade, C.M., Antranikian, G.,"Characterization of amylolytic and pullulytic enzymes from thermophilic archaea and from a new Fervidobacterium species.Appl. Microbiol. Biotechnol., 42, 239-245, 1994.". - 2-09.041V
Andrade, C.M., Antranikian, G.,"Screening and investigation for thermostable xylanases in thermophilic and hyperthermophilic archaea. 17th European Congress on Biotechnology, 19.-23. Februar, Nice, France, 1995.". - 2-09.042V
Prowe, S.G., Antranikian, G.,"Thermoalkalibacter bogoriae, a new anaerobic thermoalkaliphile isolated from the east african Lake Bogoria.VAAM-Tagung 26.-29.03.1995, Stuttgart.". - 2-09.043V
Friedrich, A.B., Böckle, B., Müller, R., Antranikian, G.,"Keratin degradation by Fervidobacterium pennavorans and Streptomyces pactum. Biospektrum, Sonderausgabe, 32, 1995.". - 2-09.044V
Friedrich, A.B., Hippe, H., Antranikian, G.,"Characterization of the keratinolytic enzyme system from a new thermophilic anaerobic bacterium, Fervidobacterium pennavorans. Appl. Environ. Microbiol., eingereicht.". - 2-09.045V
Klingeberg, M., Galunsky, B., Sjoholm, C., Kasche, V., Antranikian, G.,"Purification and properties of a highly thermostable, sodium dodecyl sulfate-resistant and stereospecific proteinase from the extremely thermophilic archaeon Thermococcus stetteri. Appl. Environ. Microbiol, 61, 3098-3104, 1995. - 2-09.046V
Krahe, M., G. Antranikian and H. Märkl: Fermentation of extremophilic microorganisms. FEMS Microbiology Reviews 18 (1996) 271 - 285 - 2-09.047V
Pörtner, R.; A. Schilling; I, Lüdemann and H. Märkl: High density fed-batch cultures for hybridoma cells performed with the aid of a kinetic model. Bioprocess Engineering 15 (1996) 117-124 - 2-09.048V
Lüdemann, I., R. Pörtner, C. Schaefer, K. Schick, K. Srámková, K. Reher, M. Neumaier, F. Franék and H. Märkl: Improvement of the culture stability of non-anchorage-dependent animal cells grown in serum-free media through immobilization. Cytotechnology 19 (1996) 111-124 - 2-09.049V
Reinhold, G., S. Merrath, F. Lennemann and H. Märkl: Modelling the hydrodynamics and the fluid mixing behaviour of a Biogas Tower Reactor. Chem. Eng. Sci. 51,17 (1996) 4065-4073 - 2-09.050V
Pörtner, R., I. Lüdemann und H. Märkl: Dialysekulturen mit immobilisierten tierischen Zellen. Biospektrum 6 (1996) 47-49 - 2-09.051V
Pörtner, R.; I. Lüdemann and H. Märkl: Dialysis cultures with immobilized hybridoma cells for effective production of monoclonal antibodies. Cytotechnology 23: 39-45 (1997) - 2-09.052V
Reinhold, G. and H. Märkl: Model based scale up and performance of the Biogas Tower Reactor for anaerobic waste water treatment. Wat. Res. 31, 8: 2057-2065 (1997) - 2-09.053V
Nakano, N.; M. Rischke, S. Sato and H. Märkl: Influence of acetic acid on the growth of Escherichia coli K12 during high-cell-density cultivation in a dialysis reactor. Appl. Microbiol. Biotechnol. 48: 597-601 (1997) - 2-09.054V
Becker, P.; I. Abu-Reesh; S. Markossian; G. Antranikian and H. Märkl: Determination of the kinetic parameters during continuous cultivation of the lipase-producing thermophile Bacillus sp. IHI-91 on olive oil. Appl. Microbiol. Biotechnol. 48:184-190 (1997) - 2-09.055V
Pörtner, R.; I. Lüdemann; K. Reher; M. Neumaier; H. Märkl:(1998) Fixed-bed dialysis culture of a transfectoma cell line producing chimeric Fab-fragments with "nutrient-split"-feeding strategy. Biotechnol. Tech. 12,7: 501-505 (1998) - 2-09.056V
Schwabe, J.O.; R. Pörtner and H. Märkl: Improving an on-line feeding strategy for fed-batch cultures of hybridoma cells by dialysis and "nutrient-split"-feeding. Bioproc. Eng. 20, 6: 475 - 484 (1999) - 2-09.057V
Pörtner, R. and Th. Schäfer: Modelling hybridoma cell growth and metabolism - A comparision of selected models and data; Journal of Biotechnology 49: 119-135 (1996) - 2-09.058V
Pörtner, R. and H. Märkl: Dialysis cultures. Appl. Microbiol. Biotechnol. 50: 403-414 (1998) - 2-09.059V
Fassnacht, D.; S. Rössing; N. Ghaussy and R. Pörtner: Influence of non-essential amino acids on apoptotic and necrotic death of mouse hybridoma cells in batch cultures. Biotechnology Letters 19: 35-38 (1997) - 2-09.060V
Pörtner, R.; S. Rössing; M. Koop and I. Lüdemann: Kinetic studies on hybridoma cells immobilized in fixed bed reactos. Biotechnology and Bioengineering, 55, 3: 535-541 (1997) - 2-09.061V
Pörtner, R.; S. Rössing und D. Fassnacht: Festbettreaktoren mit immobilisierten tierischen und menschlichen Zellen. Bioscope 9: 25-33 (1997) - 2-09.062V
Pörtner, R. and M. Koop: A model for oxygen supply in fixed bed reactors with immobilized hybridoma cells. Bioprocess Engineering 17: 269-275 (1997) - 2-09.063V
Fassnacht, D.; S. Rössing; J. Stange and R. Pörtner: Long-term cultivation of immortalised mouse hepatocytes in a high cell density fixed bed reactor. Biotechnology Techniques 12: 25-30 (1998) - 2-09.064V
Fassnacht, D.; S. Rössing; F. Franék; M. Al-Rubeai and R. Pörtner: Effect of BCL-2 Expression on hybridoma cell growth in serum-supplemented, protein-free and diluted media. Cytotechnology 26 (3): 219-225 (1998) - 2-09.065V
Fassnacht, D.; S. Rössing; R. Singh; M. Al-Rubeai and R. Pörtner: Influence of BCL-2 Expression on antibody productivity in high cell density hybridoma culture systems. Cytotechnology 30: 95-105 (1999) - 2-09.066V
Fassnacht, D. and R. Pörtner: Experimental and theoretical considerations on oxygen supply for animal cell growth in fixed bed reactors. Journal of Biotechnology 72 (3): 169 - 184 (1999) - 2-09.067V
Manusadzianas, L.; R. Vitkas; R. Pörtner and H. Märkl: Phytotoxicities of selected chemicals and industrial effluents to Nitellopsis obtusa cells, assessed by using a rapid electrophysiological Charophyte test. ATLA, 27: 379-386 (1999) - 2-09.068V
Nehring, D., P. Adamietz, N. M. Meenen and R. Pörtner: Perfusion cultures and modelling of oxygen uptake with three-dimensional chondrocyte pellets. Biotechnology Techniques 13: 701-706 (1999) - 2-09.069V
Pörtner, R.; D. Fassnacht and H. Märkl: Immobilization of mammalian cells in fixed bed reactors. BIOforum International 3: 140-141 (1999) - 2-09.070V
Fussenegger, M.; D. Fassnacht; R. Schwartz; J. A. Zanghi; M. Graf, J. E. Bailey and R. Pörtner: Regulated overexpression of the survival factor bcl-2 in CHO cells increases viable cell density in batch culture and decreases DNA release in extended fixed-bed cultivation. Cytotechnology 32: 45-61 (2000) - 2-09.071V
Märkl, H., Pietsch, T.: Mathematical Modelling and Kinetics of Anaerobic Digestion, erschienen in: Technik anaerober Prozesse. Dechema-Fachgespräche Umwelt-schutz (1998), S.69-82 - 2-09.072V
Märkl, H.: Modeling of biogas reactors. In: H.-J. Rehm and G. Reed: Biotechnology, Wiley-VCH, Weinheim, Volume 11, pp. 529-560 - 2-09.073V
Bertoldo C, Duffner F, Jørgensen PL, Antranikian G (1999) Pullulanase type I from Fervidobacterium pennavorans Ven5: cloning, sequencing, expression of the gene and biochemical characterization of the recombinant enzyme. Appl Environ Microbiol. 65, p 2084-2091 - 2-09.074V
Duffner, F., Bertoldo, C., Andersen, Jens T., Wagner, K., and Antranikian G. (2000) A new thermoactive pullulanase from Desulfurococcus mucosus: cloning, sequencing, purification and characteriaztion of the recombinant enzyme after expression in Bacillus subtilis. J. Bacteriol. 182, 6331-6338 - 2-09.075V
Bohlke K; Pisani FM; Vorgias CE; Frey B; Sobek H; Rossi M; Antranikian G (2000) PCR performance of the B-type DNA polymerase from the thermophilic euryarchaeonthermococcus aggregans improved by mutations in the Y-GG/A motif Nucleic Acids Res, 28, 3910-3917 - 2-09.076V
Niehaus F; Peters A; Groudieva T; Antranikian G (2000) Cloning, expression and biochemical characterisation of a unique thermostable pullulan-hydrolysing enzyme from the hyperthermophilic archaeon Thermococcus aggregans FEMS Microbiol Lett 190:223-229 - 2-09.077V
Grote, R., Li, L., Tamaoka, J., Kato, Chiaki, Horikoshi, K. and Antranikian, G. (1999) Thermococcus siculi sp. nov., a novel hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent at the Mid-Okinawa Trough. Extremophiles 3:55-62 - 2-09.078V
Frillingos S, Linden A, Niehaus F, and Antranikian G. (2000) Cloning and expression of alpha-amylase from the hyperthermophilic archaeon Pyrococcus woesei in the moderately halophilic bacterium Halomonas elongata. J Appl Microbiol., 88, 495-503 - 2-09.079V
Linden A, Niehaus F, Antranikian G. (2000) Single-step purification of a recombinant thermostable alpha-amylase after solubilization of the enzyme from insoluble aggregates. J Chromatogr B Biomed Sci Appl 737, 253-9 - 2-09.080V
Niehaus F, Bertoldo C, Kahler M, Antranikian G. (1999) Extremophiles as a source of novel enzymes for industrial application. Appl Microbiol Biotechnol. 51: 711-729 - 2-09.081V
Stefanova ME; Schwerdtfeger R; Antranikian G; Scandurra R. (1999) Heat-stable pullulanase from Bacillus acidopullulyticus: characterization and refolding after guanidinium chloride-induced unfolding. Extremophiles:147-52 - 2-09.082V
Markossian, S.; P. Becker, H. Märkl and G. Antranikian: Isolation and characterization of lipid-degrading Bacillus thermoleovorans IHI-91 from an icelandic hot spring. Extremophiles 4: 365-371 (2000) - 2-09.083V
Becker, P. and H. Märkl: Modeling of olive oil degradation and oleic acid inhibition during chemostat and batch cultivation of Bacillus thermoleovorans IHI-91. Biotechnology and Bioengineering, 70,6: 632-637 (2000) - 2-09.084V
Feitkenhauer, H.; S. Schnicke, R. Müller and H. Märkl: Determination of kinetic parameters of the pheol-degrading thermophile Bacillus thermoleovorans sp. A2. Applied Microbiology and Biotechnology 57: 744-750 (2001) - 2-09.085V
Reimann, I. und H. Märkl (2001): Thermophile Mikroorganismen für die Reinigung fetthaltiger Abwässer. Sonderband Biokatalyse der DBU - 2-09.086V
Feitkenhauer, H.; S. Schnicke, R. Müller and H. Märkl (2001): Determination of kinetic parameters of the phenol-degrading thermophile Bacillus thermoleovorans sp. A2. Applied Microbiology and Biotechnology 57: 744-750 - 2-09.087V
Fuchs, C.; D. Köster, S. Wiebusch, K. Mahr, G. Eisbrenner, H. Märkl (2002): Scale up of the dialysis fermentation for high cell density fermentation of E. coli. Journal of Biotechnology 93, 3, 243-251 - 2-09.088V
Biller, F. K.; I. Kato, H. Märkl (2002): Effect of glucose, maltose, soluble starch, and CO2 on the growth of the hyperthermophilic archaeon Pyrococcus furiosus. Extremophiles 6: 161-166 - 2-09.089V
Reimann, I.; A. Klatt, H. Märkl (2002): Behandlung fetthaltiger Abwässer der Lebensmittelindustrie mit einem thermophilen Mikroorganismus, Chemie Ingenieur Technik 74: 508-512 - 2-09.090V
Pietsch, T.; R. Mehrwald, R. Grajetzki, J. Sens, T. Pakendorf, R. Ulrich, J. Kumpart, G. Matz, H. Märkl (2002): Macro- and microscopic in-situ observation of gas bubbles and sludge particles in a biogas tower reactor. Water Research 36: 2836-2842 - 2-09.091V
Pietsch, T.; Mehrwald, R.; Grajetzki, R.; Sens, J.; Märkl, H. (2003): Sedimentation behaviour of sludge particles in a biogas tower reactor and the function of a hydrostatically pressurized sedimenter. Water Research 37 (5):1071-1079. - 2-09.092V
Fassnacht, D.; I. Reimann, R. Pörtner und H. Märkl: Scale-up von Festbettreak-toren zur Kultivierung tierischer Zellen. Chemie Ingenieur Technik 73: 1075 - 1079 (2001) - 2-09.093V
Frahm, B.; H.-C. Blank; P. Cornand; W. Oelßner; U. Guth; P. Lane; A. Munack; K. Johannsen and R. Pörtner: Determination of dissolved CO2 concentration and CO2 production rate of mammalian cell suspension culture based on off-gas measurement. Journal of Biotechnology 99/2: 133-148 (2002) - 2-09.094V
Frahm, B.; P. Lane; H. Atzert; A. Munack; M. Hoffmann; V. Hass and R. Pörtner: Adaptive, model-based control by the Open-Loop-Feedback-Optimal (OLFO) controller for the effective fed-batch cultivation of hybridoma cells. Biotechnology Progress 18: 1095-1103 (2002) - 2-09.095V
Nagel-Heyer, St. and R. Pörtner: Bioreactor cultivation for three-dimensional cartilage implants. G.I.T. Laboratory Journal 2: 66-67 (2002) - 2-09.096V
Frahm, B. und R. Pörtner: Reaktor-Abgasmessungen bei Zellkulturen, BIOforum 4, 2002 - 2-09.097V
Reimann, I.; A. Klatt, H. Märkl (2002): Treatment of Wastewater Containing Fat from Food Industry with a Thermophilic Microorganism. Engineering in Life Sciences 2, 12:398-401. - 2-09.098V
Nehring, D.; P. Czermak; S. Gokorsch und R. Pörtner: Produktion retroviraler Partikel im Festbettreaktor. BioWorld 4: 14-15 (2003) - 2-09.099V
Frahm, B., Hass, V. C., Lane, P., Munack, A., Märkl, H. and R. Pörtner: Fed-batch Kultivierung tierischer Zellen - eine Herausforderung zur adaptiven, modellbasierten Steuerung, Chemie Ingenieur Technik, vol. 4, pp. 457 460 (2003) - 2-09.100V
Frahm, B., Lane, P., Märkl, H. and R. Pörtner: Improvement of a mammalian cell culture process by adaptive, model-based dialysis fed-batch cultivation and suppression of apoptosis, Bioprocess and Biosystems Engineering, vol. 26, pp. 1-10 (2003) - 2-09.101V
Krüger, M., Reimann, I., Märkl, H., Taube, J., Eckert, W. (2003): Aerobe thermophile Reinigung fetthaltiger Abwässer der Lebensmittelindustrie mit Bacillus thermoleovorans, Trankscript Sonderband 2003, 20-23 - 2-09.102V
Brodersen, J., Rießen, S., Antranikian, G., Märkl, H. (2003): Umweltfreundliche Aufbereitung von Hühnerfedern mittels extremthermophiler Bakterien und thermostabilen Enzymen, Trankscript Sonderband 2003, 17-19 - 2-09.103V
Nehring, D.; R. Gonzales; P. Czermak and R. Pörtner: Mathematical model of a mem-brane filtration process using ceramic membranes to increase retroviral pseudotype vector titer. Journal of Membrane Science 237: 25-38 (2004) - 2-09.104V
Pörtner, R.; J.-O. Schwabe and B. Frahm: Evaluation of selected control strategies for fed-batch cultures of a hybridoma cell line, Biotechnology and Applied Biochemistry 40: 47-55 (2004) - 2-09.105V
Nagel-Heyer, S.; R. Janßen; C. Goepfert; P. Adamietz; N.M. Meenen and R. Pörtner: Carti-lage cultivation on a calcium phosphate ceramic in a bioreactor system. Key Engineering Materials 264-268: 2119-2122 (2004) - 2-09.106V
Janssen, R.; S. Nagel-Heyer; Ch. Goepfert; R. Pörtner; D. Toykan; O. Krummhauer; M.M. Morlock; P. Adamietz; N.M. Meenen; W.M. Kriven; D.-K. Kim; A. Tampieri and G. Celotti: Cal-cium phosphate ceramics as substrate for cartilage cultivation. Ceramic Engineering and Sci-ence Proceedings 25, 4: 523-528 (2004) - 2-09.107V
Pörtner, R.; M. Seemuk; R.-Ch. Schlothauer; D. Elsser: Anaerobe Kultivierung von Lactococcus lactis im Festbettreaktor. Chemie-Ingenieur-Technik, 76, 10: 1599-1602 (2004) - 2-09.108V
Nagel-Heyer, S.; Ch. Goepfert; M.M. Morlock and R. Pörtner: Relationship between gross morphological and biochemical data of tissue engineered cartilage-carrier-constructs. Biotechnology Letters, 27(3): 187-192 (2005) - 2-09.109V
Kasche, V.; Z. Ignatova; H. Markl; W. Plate; N. Punckt; D. Schmidt; K. Wiegandt and B. Ernst: Ca2+ is a cofactor required for membrane transport and maturation and is a yield determining factor in high cell density penicillin amidase production. Biotechnol Prog 21(2):432-8 (2005) - 2-09.110V
Nagel-Heyer, S.; Ch. Goepfert; M.M. Morlock and R. Pörtner: Relationship between gross morphological and biochemical data of tissue engineered cartilage-carrier-constructs. Biotechnology Letters 27(3): 187-192 (2005) - 2-09.111V
Frahm, B.; P. Lane, A. Munack und R. Pörtner: Optimierung und Steuerung von Zellkultur-Fed-Batch-Prozessen mittels einer Kollokationsmethode. Chemie-Ingenieur-Technik, 77 (4), 429 – 435 (2005) - 2-09.112V
Nagel-Heyer, S.; Ch. Goepfert; P. Adamietz; N.M. Meenen; J.-P. Petersen and R. Pörtner: Flow-chamber bioreactor culture for generation of three-dimensional cartilage-carrier-constructs. Bioprocess and Biosystems Engineering, 27: 273-280 (2005) - 2-09.113V
Meenen, N.M., P. Ueblacker, R. Pörtner, C. Göpfert, S. Nagel-Heyer, J. P. Petersen und P. Adamietz: ; „ Knorpel aus dem Labor-eine Sackgasse?“,. Arthroskopie 18: 245-252 (2005) - 2-09.114V
Pörtner, R.; St. Nagel-Heyer; Ch. Goepfert; P. Adamietz and N. M. Meenen: Bioreactor design for tissue engineering. Journal of Bioengineering and Bioscience, 100(3): 235-245 (2005)
Dissertationen
- 2-09.001D
Reinhold, Georg: Maßstabsvergrößerung eines Biogas-Turmreaktors zur anaeroben Abwasserreinigung, 1996 - 2-09.002D
Lüdemann, Ines: "Nutrient-Split"-Fütterungsstrategie für suspendierte und immobilisierte nicht-adhärente tierische Zellen, 1997 - 2-09.003D
Krahe, Martin: Kultivierung von Mikroorganismen zu hohen Zelldichten, 1997. - 2-09.004D
Müller, Birgit: Untersuchungen zum Abbau polyzyklischer aromatischer Kohlenwasserstoffe (PAK) durch thermophile Mikroorganismen, 1998. - 2-09.005D
Polomski, Andreas: Einfluß des Stofftransports auf die Methanbildung in Biogas-Reaktoren, 1998 - 2-09.006D
Feitkenhauer, Heiko: Biodegradation of aliphatic and aromatic hydrocarbons at high temperatures, 1998 - 2-09.007D
Pietsch, Torsten: Rückhalt suspendierter Biomasse und örtliche Verteilung gelöster Gase in einem Biogas-Turmreaktor, 2000 - 2-09.008D
Becker, Peter: Der biologische Abbau von Fetten durch aerobe, thermophile Bakterien: Untersuchungen zur Kinetik und zum Einsatz in der Abwassertechnik, 1999 - 2-09.009D
Fassnacht, Dieter: Fixed-bed reactors for the cultivation of animal cells, 2000 - 2-09.010D
Schwabe, Jan-Oliver: Feeding Strategies for Fed-Batch Cultures of Animal Cells, 2000 - 2-09.011D
Niehaus, F.: Klonierung und Charakterisierung einer neuen, thermostabilen a-DNA Polymerase, 1996 - 2-09.012D
Prowe, S.: Characterization of Extracellular Amylolytic Enzymes and Sodium Coupled Energy Transduction of a Newly Isolated Thermoalkaliphilic Bacterium, Thermoalcalibacter bogoriae, 1996 - 2-09.013D
Schwerdtfeger, R.: Structure/Function Relationships of Pullulanase and Glutamate Dehydrogenase from the Hyperthermophilic Archaeon Pyrococcus woesei, 1997 - 2-09.014D
Hebenbrock, S.: Isolierung und Charakterisierung von thermophilen Mikroorganismen und deren Untersuchungen zum mikrobiellen Abbau von Naphthalin und Benzoesäure, 1998 - 2-09.015D
Reinigung und biochemische Charakterisierung einer thermostabilena-Amylase aus Pyrococcus woesei nach Klonierung und Expression inEscherichia coli und Halomonas elongata. 1999 - 2-09.016D
Kähler M. Cloning and characterization of DNA polymerase from the hyperthermophilic Crenoarchaeon Pyrobaculum islandicum. 1999 - 2-09.017D
Serour E., Production, Purification and characterization of novel thermoactive glucoamylases from hyperacidophilic archaea Thermoplasma acidophilum, Picrophilus torridus and Picrophilus oshimae. 2000 - 2-09.018D
Fuchs, C.: Einsatz einer Dialysemembran bei der Fermentation von E. coli. 2002 - 2-09.019D
Köster, D.: Proteinexpression in dichten Kulturen von E. coli. 2002 - 2-09.020D
Biller, K.: Fermentation hyperthermophiler Mikroorganismen. 2002 - 2-09.021D
Grote R., Thermococcus siculi sp. nov., a novel hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent at the Mid-Okinawa Trough. Extremophiles 3:55-62. (1999) - 2-09.022D
Hansen, G.: Der Venturi-Bioreaktor: Ein System mit hoher Stoffaustauschleistung zwischen Gas- und Flüssigphase. 2001 - 2-09.023D
Reimann, I.: Thermophile Behandlungsverfahren zur biologischen Reinigung fetthaltiger Abwässer. 2003 - 2-09.024D
Frahm, B.: Adaptive, modellgestützte Prozessführung von Suspensionskulturen tierischer Zellen. 2003 - 2-09.025D
Nagel-Heyer, St.: Ingenieurtechnische Aspekte bei der Herstellung von dreidimensionalen Knorpel-Träger-Konstrukten. 2004 - 2-09.026D
Brodersen, J.: Biotechnologische Verfahren zum Abbau von Federn - 2-09.027D
Göpel, Ph.: Pektinolytische Enzyme aus thermophilen Bakterien: Wirkungsweise auf Bastfasern, Charakterisierung und Produktion
Habilitationen
- 2-09.001H
Pörtner, R.: Reaktionstechnik der Kultur tierischer Zellen. 1997