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Fluorescence thermometer

Description of the company
Eppendorf is a renowned German company specializing in the development, manufacture, and distribution of high-quality laboratory equipment and consumables. Founded in 1945 by Dr. Heinrich Netheler and Dr. Hans Hinz in Hamburg, Germany, the company has since become a global leader in the life sciences industry. Eppendorf's product portfolio includes laboratory instruments such as centrifuges, pipettes, shakers, and incubators, as well as consumables like tubes, plates, and tips.

With a strong focus on innovation, Eppendorf continuously introduces new technologies and solutions to meet the evolving needs of researchers and scientists worldwide. All products are known for their reliability, precision, and user-friendly design, making them essential tools in various fields including molecular biology, cell biology, microbiology, and biotechnology.

Eppendorf's commitment to quality and customer satisfaction has earned them a trusted reputation within the scientific community. Through their global network of subsidiaries, distributors, and service centers, they provide comprehensive support and assistance to customers around the world, helping to advance scientific research and discovery.


An accurate pipetting result is one of the most important factors in most laboratory applications. The temperature of a liquid plays a decisive role in laboratory experiments
Traditional thermometers are often used to measure the temperature of a liquid directly.


Especially with very small liquid sample quantities and in systems that are difficult to access, measuring the actual temperature in the sample is often difficult, inaccurate or time-delayed.

Aims & Scope of the project

The principle of fluorescence-based temperature measurement is to be demonstrated by means of an experimental setup ("proof of concept"). This requires the use of suitable optical measuring systems.
•    The exact absolute temperature can be determined and stored using a calibration curve.
•    In the subsequent optimization step, the minimum required quantities of the necessary fluorophores, the appropriate excitation light intensity, usable temperature ranges are to be determined.
•    Finally, it will be investigated how the fluorophores can best be used in practice, i.e. how they can be easily portioned and brought to the desired measuring location.

Target group (students)

Study Programs:
•    Chemical Engineering
•    Bioengineering
•    Process Engineering
•    Technical Chemistry
•    Biotechnology
•    Mechanical Engineering with a focus on instrumentation or sensor technology
•    Electrical Engineering with a focus on sensor technology or signal processing

•    Proficiency in chemistry and biochemistry
•    Experience in handling laboratory equipment and techniques, especially in sample preparation and analysis
•    Familiarity with fluorescence phenomena and measurements

•    Interest in precision measurement techniques and laboratory technologies
•    Curiosity about innovative approaches to temperature measurement in laboratories
•    Enthusiasm for the application of fluorescence in engineering and scientific contexts

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