Sebastian Hofmann, M.Sc.

Eißendorfer Str. 38, Building O, Room 1.013

Telephone +49 40 42878-3106

E-Mail: Sebastian Hofmann, M.Sc.


Research

DFG Priority Program SPP2170 "InterZell"

 

CHOLife: Multiscale experimental analysis and simulation of lifelines in bioreactors to study their impact on the cultivation performance of Chinese Hamster Ovary (CHO) cells.

For the detailed understanding of cultivation processes it is indispensable to get deep insights into the overall and local hydrodynamics with high spatial and temporal resolution because velocity gradients and mixing are dominating concentration gradients (pH, O2, CO2), shear-stress as well as the mass transfer performance. 

CHOLife tackles the scale-up problem linking the unique access to a 12,000 L cell culture bioreactor (Institute of Multiphase Flows, TUHH Hamburg, IMS) with the development of a novel scale-up device (Institute of Biochemical Engineering, Stuttgart, IBVT) finally to predict industrial scale performance of IgG1 producing CHO cells. In essence, three dimensional flow trajectories and lifelines will be measured at IMS and mimicked in the novel scale-up simulator of IBVT. The close cooperation of both parties ensures a successful scale-down of large scale conditions and an equally successful prediction of large-scale performance of CHO cells cultured in the novel scale-up simulator.

The left image below depicts the experimental setup for 4D-Particle Imaging Velocimetry (4D-PTV) measurements at the Institute of Multiphase Flows, whereas C1-C4 mark the high-speed cameras (two Phantom VEO 640L and two PCO dimax) and L1-L3 show the UV light sources for the fluorescent particles inside the 3 L reactor. The right image shows some exemplary particle trajectories.


Science Communication

Füchse der Wissenschaft
  • In the seventh episode of "Füchse der Wissenschaft" Jan and Sebastian invited two new PhD students from the TU Berlin, namely Karin and Tolue. They talk about how to stay motivated, why they use the fungi Aspergillus niger in their [...]
  • Was ist das Geheimnis des mRNA Impfstoffs "BNT162b2" von BioNTech? Warum kann man diesen als "Code" bezeichnen und wie ist er aufgebaut? Wo kann man außerdem Details zur Zulassung des Impfstoffs von Astra Zeneca nachlesen? Diese und noch [...]
  • The fifth episode focuses on how two different kind of microorganisms (fungi and bacteria) can be used together in order to utilize the abundant resource cellulose as a substrate in a co-culture. Ana and Maurice, from Jena and Aachen, [...]
  • In der vierten Episode erklären Martina und Miriam von der TU München wie das Bakterium Clostridium in der Lage ist mit Abgas wertvolle Produkte herzustellen. Im Projekt SynCoClos geht es dabei um einen umweltfreundlichen Ansatz zur [...]
  • Third episode of "Füchse der Wissenschaft" - finally something in English, for everyone being curious what the two hosts Sebastian and Jan are actually doing here. This time, it's about the topic gene editing and obviously the sub-project [...]
  • Für die zweite Folge "Füchse der Wissenschaft" haben Sebastian und Jan die zwei Doktoranden Tobi und Simon aus der Uni Stuttgart eingeladen. Wie es sich im "Kessel" lebt, warum E. coli der ideale Mikroorganismus ist und was hinter dem Namen [...]
  • In der ersten Folge von "Füchse der Wissenschaft" sprechen Sebastian und Jan mit Franzi von der Technischen Universität München, über ihre ungewöhnliche Laufbahn, ihre Vorliebe für langjährige Planungen und Fortschritte im Teilprojekt.

     [...]
  • Wie funktioniert Biotech, was ist das eigentlich, wie klappt so eine Promotion auf diesem vielfältigen Gebiet und was hat das Feierabendbierchen mit Biotechnologie zu tun... Das und noch viel mehr erfahrt ihr hier bei uns.

    "Füchse der [...]


Awards

  • Winner of the I3 Junior Project: Flow-following Lagrangian Sensor Particles - Development of a novel measurement technique with conductivity sensors for a novel macro-mixing and lifeline analysis; Hofmann, S.; Kamp, M.; Buntkiel, L.; Reinecke, S.; I3-Programm, sponsored by the Hamburg University of Technology for the project period 01/2023 - 03/2024


Education

Graduate Teaching Assistent

  • Reactor Design Using Local Transport Processes (Winter Semester 2022/23)
  • Reactor Design Using Local Transport Processes (Winter Semester 2021/22)
  • Grundlagen des Technischen Zeichnens (Summer Semester 2021)


Supervised Theses

  • "Process characterization in stirred tank bioreactors by means of Lagrangian Sensor Particles and evaluation of their flow-following capability", Ryan Rautenbach, Master's Thesis, 2022-2023
  • "Experimental investigation of the flow behavior of Lagrangian LED Particles in a 200 l bioreactor", Maximilian Kamp, Bachelor's Thesis, 2021-2022: Thesis available here and MATLAB code available here.
  • "Design, Construction and Evaluation of Lagrangian Sensor Particles for the Flow Behavior Determination in a 200 L and 15000 L Bioreactor", Paramveer GopalSingh, Master's Thesis, 2021-2022 (still ongoing)

  • "Experimental Characterization of Modified Polyurethane Foams for Mechanical Clean Up of Oil Pollutants at Sea Surface" (in close cooperation with Daniel Niehaus), Srividya Kumar Bairamangala, Project Work, 2020-2021

 


Oral and Poster Presentations

  • Hofmann, S.; Weiland, C.; GopalSingh, P.; Kamp, M.; Fitschen, J.; v. Kameke, A.; Hoffmann, M.; Schlüter, M.: Experimental and Numerical Determination of Lifelines in a 3 L, 200 L and 15000 L Stirred Tank Reactor to Estimate the Flow-Following Capability of Lagrangian Sensor Particles, FTZ 3i Talk: Conference on intelligent - industrial - innovations, HAW Hamburg 2022, Poster Presentation
  • Hofmann, S.; Gaugler, L.: CHOLife - Multiscale Experimental Analysis and Simulation of Lifelines in Bioreactors to Study their Impact on the Cultivation Performance of CHO Cells, Annual Meeting: Priority Program Status Meeting of the SPP2170 "InterZell", organized by Uni Stuttgart, Stuttgart, 2022, Presentation
  • Hofmann, S.; Weiland, C.; GopalSingh, P.; Kamp, M.; Fitschen, J.; v. Kameke, A.; Hoffmann, M.; Schlüter, M.: Experimental and Numerical Determination of Lifelines in a 3 L, 200 L and 15000 L Stirred Tank Reactor to Estimate the Flow-Following Capability of Lagrangian Sensor Particles, 4th International Symposium on Multiscale Multiphase Process Engineering (MMPE), Berlin 2022, Poster Presentation
  • Fitschen, J.; Kuschel, M.; Hofmann, S.; Wucherpfennig, T.; v. Kameke, A.; Hoffmann, M.; Schlüter, M.: Characterization of Heterogeneities in Stirred Tank Reactors by Means of 4D Particle Trajectories and Numerical Flow Simulations, MIXING XXVII, North American Mixing Forum (NAMF), Online Conference 2022, Presentation
  • Hofmann, S.; Vernier-Lambert, H.; Meriguet, S.; GopalSingh, P.; Fitschen, J.; Neubauer, P.; von Lieres, E.; Ferguson, M.; Schlüter, M.: Assessment of Lagrangian Sensor Particle Designs in a Transparent 15,000 L Acrylic Glass Bioreactor, 7th BioProScale Symposium, organized by Prof. Neubauer (TU Berlin), Langenbeck-Virchow-Haus, Berlin 2022, Presentation
  • Gaugler, L.; Mast, Y.; Fitschen, J.; Hofmann, S.; Schlüter, M.; Takors, R.: Development of a single multi-compartment bioreactor (SMCB) for CHO scale-down studies in a heterogeneous cultivation environments, 7th BioProScale Symposium, organized by Prof. Neubauer (TU Berlin), Langenbeck-Virchow-Haus, Berlin 2022, Presentation
  • Weiland, C; Hofmann, S.; Fitschen, J.; von Kameke, A.; Hoffmann, M.; Schlüter, M.: Numerische Simulation Lagranger Partikeltrajektorien und Charakterisierung des Partikelfolgevermögens in einem 3 L Rührkesselreaktor, Jahrestreffen der ProcessNet-Fachgruppen Computational Fluid Dynamics + Mischvorgänge + Agglomerations- und Schüttguttechnik, DECHEMA, Hyperion Hotel Leipzig, 2022, Presentation
  • Fitschen, J.; Hofmann, S.; Hoffmann, M.; Kuschel, M.; Wucherpfennig, T.; Schlüter, M.: Lokale Mischzeitverteilung in begasten Rührkesselreaktoren im Labormaßstab, Jahrestreffen der ProcessNet-Fachgruppen Computational Fluid Dynamics + Mischvorgänge + Agglomerations- und Schüttguttechnik, DECHEMA, Hyperion Hotel Leipzig, 2022, Presentation
  • Hofmann, S.; Weiland, C.; Fitschen, J.; v. Kameke, A.; Hoffmann, M.; Schlüter, M.: Vergleich von experimentellen und numerischen Untersuchungen zur Abschätzung des Folgevermögens verschiedener Lagrange’scher Partikel in der Bioreaktorströmung, Jahrestreffen der ProcessNet-Fachgruppen Computational Fluid Dynamics + Mischvorgänge + Agglomerations- und Schüttguttechnik, DECHEMA, Hyperion Hotel Leipzig, 2022, Presentation
  • Hofmann, S.; Gaugler, L.; Fitschen, J.; v. Kameke, A.; Schlüter, M.; Takors, R.: Lagrangian Particle Tracking and Bioreactor Compartmentalization as Novel Scale-up Tools for Biopharmaceutical Processes, 13th European Congress of Chemical Engineering, 6th European Congress of Applied Biotechnology (ECCE 13 & ECAB 6), DECHEMA, Online conference, 2021, Presentation
  • Fitschen, J.; Kuschel, M.; Hofmann, S.; Hoffmann, H.; von Kameke, A.; Wucherpfennig, T.; Wutz, J.; Schlüter, M.: Validation of Numerical Flow Simulations in a 3 L Stirred Tank by Means of Real 4D Particle Trajectories 13th European Congress of Chemical Engineering, 6th European Congress of Applied Biotechnology (ECCE 13 & ECAB 6), DECHEMA, Online conference, 2021, Presentation
  • Fitschen, J.; Hofmann, S.; Hoffmann, H.; von Kameke, A.; Schlüter, M.: Measurement of Lagrangian Tracks in a 3 L Stirred Tank Reactor using 4D Particle Tracking Velocimetry with Shake-the-Box, ISPIV 21 (virtual), 2021, Presentation
  • Fitschen, J.; Kuschel, M.; Hofmann, S.; Hoffmann, H.; von Kameke, A.; Wucherpfennig, T.; Wutz, J.; Schlüter, M.: Validierung von numerischen Strömungssimulationen in einem 3L Rührkesselreaktor mittels 3D Partikel Trajektorien, Jahrestreffen der ProcessNet-Fachgruppen Computational Fluid Dynamics und Mehrphasenströmungen, 2021, Plenary Lecture
  • Hofmann, S.; Gaugler, L.: CHOLife - Multiscale Experimental Analysis and Simulation of Lifelines in Bioreactors to Study their Impact on the Cultivation Performance of CHO Cells, 2nd Annual Priority Program Status Meeting of the SPP2170 "InterZell", organized by Uni Stuttgart, Koblenz, 2021, Presentation

 

Publications

2022

  • Hofmann, S.; Weiland, C.; Fitschen, J.; von Kameke, A., Hoffmann, M.; Schlüter, M. (2022). Lagrangian sensors in a stirred tank reactor: Comparing trajectories from 4D-Particle Tracking Velocimetry and Lattice-Boltzmann simulations. Chemical Engineering Journal. 449. [Abstract] [doi]

  • Gaugler, L; Mast, Y.; Fitschen, J.; Hofmann, S.; Hoffmann, M.; Schlüter, M. (2022). Scaling-down biopharmaceutical production processes via a single multi-compartment bioreactor (SMCB). Engineering in Life Sciences. 12 [Abstract] [doi]

2021

  • Fitschen, J.; Hofmann, S.; Wutz, J.; Kameke, A. v.; Hoffmann M.; Wucherpfennig T.; Schlüter, M. (2021). Novel Evaluation Method to Determine the Local Mixing Time Distribution in Stirred Tank Reactors. Chemical Engineering Science: X. 10. [Abstract] [doi]

2016

  • Costa, L.I.; Tancini, F.; Hofmann, S.; Codari, F.; Trommsdorff, U.; (2016). From Laboratory to Industrial Continuous Production of Polylactic Acid with Low Residual Monomer. Chemical Engineering & Technology. 39. 2117-2125 [Abstract] [doi]