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Abstract: This study analyzes trajectories of three particle types in an industrial-scale bioreactor, equipped with a Rushton turbine and a pitched blade turbine, to characterize hydrodynamic compartments. The trajectories obtained from measurements with Lagrangian Sensor Particles (LSP,exp) are compared to those generated by Lattice-Boltzmann large eddy simulations (LB LES). Massless tracer particles are included as a benchmark. Discrepancies between experiments and simulations arise from differences in mass distribution, density, particle count, and particle-to-grid ratio. Increased simulated particle volume fraction causes collisions, clustering, and reduced turbulent kinetic energy. Circulation and residence time analyses reveal three hydrodynamic compartments, confirmed by local mixing time distributions. The ratio between average circulation time and global mixing time is Θglob,95 ≈ 3.0·tcirc for LSP,exp, consistent with literature.