|Title: Segmentation of T-cells in fluorescence microscopy In Proc. IEEE Engineering in Medicine and Biology Society (EMBC'15)|
|Written by: S.-T. Antoni, A. Dabrowski, D. Schetelig, B.-P. Diercks, R. Fliegert, R. Werner, I. Wolf, A. H. Guse, A. Schlaefer|
|in: August 2015|
|Address: Milan, Italy|
Abstract: In the adaptive immune system Calcium (Ca2+) is acting as a fundamental on-switch. Fluorescence microscopy is used to study the underlying mechanisms. However, living cells introduce motion, hence a precise segmentation is needed to gain knowledge about motion and deformation for subsequent analysis of (sub)cellular Ca2+ activity. We extend a segmentation algorithm and evaluate its performance using a novel scheme.