|Title: Segmentation of T-cells in fluorescence microscopy In Proc. IEEE Engineering in Medicine and Biology Society (EMBC'15)|
|Written by: S.-T. Antoni and A. Dabrowski and D. Schetelig and B.-P. Diercks and R. Fliegert and R. Werner and I. Wolf and A. H. Guse and A. Schlaefer|
|in: August 2015|
|Address: Milan, Italy|
Abstract: In the adaptive immune system Calcium (Ca2+) is acting as a fundamental on-switch. Fluorescence microscopy is used to study the underlying mechanisms. However, living cells introduce motion, hence a precise segmentation is needed to gain knowledge about motion and deformation for subsequent analysis of (sub)cellular Ca2+ activity. We extend a segmentation algorithm and evaluate its performance using a novel scheme.