SysLogics - Systems Biology of Cell Culture for Biologics

SysLogis hat die Entwicklung und Anwendung systembiologischer Methoden zur mathematischen Modellierung und Analyse von Schlüsselkomponenten in Metabolismus und regulatorischen Prozess einer neu entwickelten humanen Zelllinien eines Industriepartners für die Wirkstoffproduktion zum Ziel. Insbesondere werden dynamische Vorgänge in Zentralmetabolismus und Zellzyklus und ihre Interaktion während Zellwachstum und Produktbildung unter verschiedenen physiologischen Bedingungen bzw. mit genetischen Modifikationen analysiert, modelliert und ggf. prädiziert.

Stichworte

• Bioreaktor
• Dynamische Modellierung
• Modellierung
• Zellkultur

Publikationen

• Liu, Yuan-Hua and Bi, Jing-Xiu and Zeng, An-Ping and Yuan, Jing-Qi: A cybernetic model to describe the dynamics of myeloma cell cultivations Applied Mathematics and Computation, 205(1): S. 84--97, 2008.
• Zhou, F and Bi, J and Zeng, A P and Yuan, J: A macrokinetic model for myeloma cell culture based on stoichiometric balance Biotechnol Appl Biochem, 46(Pt 2): S. 85--95, 2007.
• Platas, Oscar B and Jandt, Uwe and Sandig, Volker and Pörtner, Ralf and Zeng, An-Ping: An integrated synchronization approach for studying cell-cycle dependent processes of mammalian cells under physiological conditions BMC Proceedings, 7(Suppl 6): S. P16, 2013.
• Freund, Susann and Rath, Alexander and Barradas, Oscar Platas and Skerhutt, Eva and Scholz, Sebastian and Niklas, Jens and Sandig, Volker and Rose, Thomas and Heinzle, Elmar and Noll, Thomas and Pörtner, Ralf and Zeng, An Ping and Reichl, Udo: Batch-to-batch variability of two human designer cell lines: AGE1.HN and AGE1.HN.AAT ; carried out by different laboratories under defined culture conditions using a mathematical model. [].
• Schrader, E and Scholz, S and Niklas, J and Rath, A and Barradas, O P and Jandt, U and Sandig, V and Rose, T and Portner, R and Reichl, U and Zeng, A P and Heinzle, E and Noll, T: Characterisation of cultivation of the human cell line AGE1.HN.AAT BMC Proc, 5 Suppl 8: S. P87, 2011.
• Platas Barradas, O and Jandt, U and Da Minh Phan, L. and Villanueva, M and Rath, A and Reichl, U and Schrader, E and Scholz, S and Noll, T and Sandig, V and Portner, R and Zeng, A P: Criteria for bioreactor comparison and operation standardisation during process development for mammalian cell culture BMC Proc, 5 Suppl 8: S. P47, 2011.
• Sercinoglu, O and Platas Barradas, O and Sandig, V and Zeng, A P and Portner, R: DoE of fed-batch processes - model-based design and experimental evaluation BMC Proc, 5 Suppl 8: S. P46, 2011.
• Platas Barradas, Oscar and Jandt, Uwe and Minh Phan, Linh Da and Villanueva, Mario E and Schaletzky, Martin and Rath, Alexander and Freund, Susann and Reichl, Udo and Skerhutt, Eva and Scholz, Sebastian and Noll, Thomas and Sandig, Volker and Pörtner, Ralf and Zeng, An-Ping: Evaluation of criteria for bioreactor comparison and operation standardization for mammalian cell culture Engineering in Life Sciences, 12(5): S. 518-528, 2012.
• Platas, Oscar B and Sandig, Volker and Pörtner, Ralf and Zeng, An-Ping: Evaluation of process parameters in shake flasks for mammalian cell culture BMC Proceedings, 7(Suppl 6): S. P17, 2013.
• Fassnacht, D and Ghaussy, N and Rössing, S and Pörtner, R: Influence of non-essential amino acids on apoptotic and necrotic death of mouse hybridoma cells in batch cultures Biotechnology Letters, 19(1): S. 35--38, 1997.
• Jandt, U and Platas Barradas, O and Portner, R and Zeng, A P: Mammalian cell culture synchronization under physiological conditions and population dynamic simulation Appl Microbiol Biotechnol, 98(10): S. 4311-4319, 2014.
• Platas Barradas, O and Jandt, U and Hass, R and Kasper, C and Sandig, V and Portner, R and Zeng, A P: Physical methods for synchronization of a human production cell line BMC Proc, 5 Suppl 8: S. P49, 2011.
• Platas Barradas, Oscar and Jandt, Uwe and Becker, Max and Bahnemann, Janina and Pörtner, Ralf and Zeng, An-Ping: Synchronized mammalian cell culture: Part I-A physical strategy for synchronized cultivation under physiological conditions Biotechnology progress, 2014.