Technologien für Zell- und Gewebekulturen

Zellkulturprozesse zur Wirkstoffproduktion

Tissue Engineering

Stichworte

• Bioreaktor
• Hochzelldichte Kultivierung
• humane Zellen
• Kinetische Modellierung
• Knorpel
• Knorpelersatz
• NS0-Zelllinien
• Stammzellen
• Tissue Engineering
• Zellkultur

Publikationen

• Pörtner, Ralf and Platas-Barradas, Oscar and Gradkowski, Janosh and Gautam, Richa and Kuhnen, Florian and Hass, Volker C: "BioProzessTrainer'' as training tool for design of experiments BMC Proceedings, 5(Suppl 8): S. P62, 2011.
• Goepfert, Christiane and Scheurer, Wibke and Rohn, Susanne and Rathjen, Britta and Meyer, Stefanie and Dittmann, Anja and Wiegandt, Katharina and Janß en, Rolf and Pörtner, Ralf: 3D-Bioreactor culture of human hepatoma cell line HepG2 as a promising tool for in vitro substance testing BMC Proceedings, 5(Suppl 8): S. P61, 2011.
• Pörtner, R and Koop, M: A model for oxygen supply in fixed bed reactors with immobilized hybridoma cells Bioprocess Engineering, 17(5): S. 269, 1997.
• Goepfert, Christiane and Blume, Grit and Faschian, Rebecca and Meyer, Stefanie and Schirmer, Cedric and Müller-Wichards, Wiebke and Müller, Jörg and Fischer, Janine and Feyerabend, Frank and Pörtner, Ralf: A modular flow-chamber bioreactor concept as a tool for continuous 2D- and 3D-cell culture BMC Proceedings, 7(Suppl 6): S. P87, 2013.
• Frahm, Björn and Lane, Paul and Atzert, Hendrik and Munack, Axel and Hoffmann, Martin and Hass, Volker C and Pörtner, Ralf: Adaptive, model-based control by the Open-Loop-Feedback-Optimal (OLFO) controller for the effective fed-batch cultivation of hybridoma cells Biotechnology progress, 18(5): S. 1095--1103, 2002.
• : Animal cell biotechnology: Methods and protocols, Jg. 1104 von Methods in molecular biology. Humana Press, New York u.a, , 3, 2014
• : Animal cell biotechnology: Methods and protocols, Jg. 24 von Methods in biotechnology. Humana Press, Totowa N.J, , 2, 2007
• Nagel-Heyer, Stephanie and Goepfert, Christiane and Feyerabend, Frank and Petersen, Jan Philipp and Adamietz, Peter and Meenen, Norbert M and Pörtner, Ralf: Bioreactor cultivation of three-dimensional cartilage-carrier-constructs Bioprocess and biosystems engineering, 27(4): S. 273--280, 2005.
• Pörtner, Ralf and Nagel-Heyer, Stephanie and Goepfert, Christiane and Adamietz, Peter and Meenen, Norbert M: Bioreactor design for tissue engineering Journal of bioscience and bioengineering, 100(3): S. 235--245, 2005.
• Kasper, Cornelia and van Griensven, Martijn and Pörtner, Ralf: Bioreactor systems for tissue engineering II: Strategies for the expansion and directed differentiation of stem cells, Jg. 123 von Advances in biochemical engineering/biotechnology. Springer, Heidelberg and New York, , 2010
• Kasper, Cornelia and van Griensven, Martijn and Pörtner, Ralf: Bioreactor systems for tissue engineering, Jg. 112 von Advances in biochemical engineering/biotechnology. Springer, Berlin, , 2008
• Pörtner, R and Hsu, H.-H and Goepfert, C: Bioreaktoren für Knochen-Tissue-Engineering Osteologie, 22(3): S. 188--195, 2013.
• Janssen, Rolf and Nagel-Heyer, Stephanie and Goepfert, Christiane and Prtner, Ralf and Toykan, Derya and Krummhauer, Oliver and Morlock, Michael and Adamietz, Peter and Meenen, Norbert M and Kriven, Waltraud M and Kim, Dong-Kyu and Tampieri, Anna and Celotti, Giancarlo: Calcium Phosphate Ceramics as Substrate for Cartilage Cultivation Ceramic Engineering and Science Proceedings , 25(4): S. 523-528, 2004.
• Nagel-Heyer, S and Janssen, Rolf and Goepfert, C and Adamietz, P and Meenen, N M and Pörtner, R: Cartilage Cultivation on a Calcium Phosphate Ceramic in a Bioreactor System Key Engineering Materials, 264-268: S. 2119--2122, 2004.
• Goepfert, C and Slobodianski, A and Schilling, A F and Adamietz, P and Pörtner, R: Cartilage engineering from mesenchymal stem cells Advances in biochemical engineering/biotechnology, 123: S. 163-200, 2010.
• Eibl, Regine and Eibl, Dieter and Pörtner, Ralf and Catapano, Gerardo and Czermak, Peter: Cell and Tissue Reaction Engineering. Springer-Verlag Berlin Heidelberg, Berlin and Heidelberg, , 2009NaNWith a Contribution by Martin Fussenegger and Wilfried Weber.
• Meyer, Stefanie and Heinsohn, Guido and Wolber, Rainer and Pörtner, Ralf and Nierle, Jens: Confocal Raman investigation of diffusion processes in monolithic type transdermal drug delivery systems Drug delivery, 2014.
• Platas Barradas, O and Jandt, U and Da Minh Phan, L. and Villanueva, M and Rath, A and Reichl, U and Schrader, E and Scholz, S and Noll, T and Sandig, V and Portner, R and Zeng, A P: Criteria for bioreactor comparison and operation standardisation during process development for mammalian cell culture BMC Proc, 5 Suppl 8: S. P47, 2011.
• Weber, Christian and Pohl, Sebastian and Pörtner, Ralf and Wallrapp, Christine and Kassem, Moustapha and Geigle, Peter and Czermak, Peter: Cultivation and Differentiation of Encapsulated hMSC-TERT in a Disposable Small-Scale Syringe-Like Fixed Bed Reactor The open biomedical engineering journal, 1: S. 64-70, 2007.
• Nagel-Heyer, Stephanie and Goepfert, Christiane and Adamietz, Peter and Meenen, Norbert M and Pörtner, Ralf: Cultivation of three-dimensional cartilage-carrier-constructs under reduced oxygen tension Journal of biotechnology, 121(4): S. 486-497, 2006.
• Schaletzky, M and Platas Barradas, O and Sievert, H and Balabanov, S and Zeng, A P and Portner, R: Cultivation strategies of a BA/F3 cell line for fundamental cell research BMC Proc, 5 Suppl 8: S. P48, 2011.
• Frahm, Björn and Blank, Heinz-Christian and Cornand, Peter and Oelssner, Wolfram and Guth, Ulrich and Lane, Paul and Munack, Axel and Johannsen, Klaus and Pörtner, Ralf: Determination of dissolved CO(2) concentration and CO(2) production rate of mammalian cell suspension culture based on off-gas measurement Journal of biotechnology, 99(2): S. 133-148, 2002.
• Pörtner, R and Märkl, H: Dialysis cultures Applied microbiology and biotechnology, 50(4): S. 403-414, 1998.
• Sercinoglu, O and Platas Barradas, O and Sandig, V and Zeng, A P and Portner, R: DoE of fed-batch processes - model-based design and experimental evaluation BMC Proc, 5 Suppl 8: S. P46, 2011.
• Lüdemann, I and Pörtner, R and Märkl, H: Effect of NH3 on the cell growth of a hybridoma cell line Cytotechnology, 14(1): S. 11-20, 1994.
• Fassnacht, D and Rössing, S and Fran\vek, F and Al-Rubeai, M and Pörtner, R: Effect of bcl-2 expression on hybridoma cell growth in serum-supplemented, protein-free and diluted media Cytotechnology, 26(3): S. 219-225, 1998.
• Blume, Grit and Müller-Wichards, Wiebke and Goepfert, Christiane and Pörtner, Ralf and Müller, Jörg: Electrical Stimulation of NIH-3T3 Cells with Platinum-PEDOT-Electrodes Integrated in a Bioreactor The open biomedical engineering journal, 7: S. 125-132, 2013.
• Pörtner, R and Bohmann, A and Lüdemann, I and Märkl, H: Estimation of specific glucose uptake rates in cultures of hybridoma cells Journal of biotechnology, 34(3): S. 237-246, 1994.
• Goepfert, Christiane and Lutz, Vivien and Lünse, Svenja and Kittel, Sabrina and Wiegandt, Katharina and Kammal, Michael and Püschel, Klaus and Pörtner, Ralf: Evaluation of cartilage specific matrix synthesis of human articular chondrocytes after extended propagation on microcarriers by image analysis The International journal of artificial organs, 33(4): S. 204--218, 2010.
• Platas Barradas, Oscar and Jandt, Uwe and Minh Phan, Linh Da and Villanueva, Mario E and Schaletzky, Martin and Rath, Alexander and Freund, Susann and Reichl, Udo and Skerhutt, Eva and Scholz, Sebastian and Noll, Thomas and Sandig, Volker and Pörtner, Ralf and Zeng, An-Ping: Evaluation of criteria for bioreactor comparison and operation standardization for mammalian cell culture Engineering in Life Sciences, 12(5): S. 518-528, 2012.
• Platas, Oscar B and Sandig, Volker and Pörtner, Ralf and Zeng, An-Ping: Evaluation of process parameters in shake flasks for mammalian cell culture BMC Proceedings, 7(Suppl 6): S. P17, 2013.
• Pörtner, Ralf and Schwabe, Jan-Oliver and Frahm, Björn: Evaluation of selected control strategies for fed-batch cultures of a hybridoma cell line Biotechnology and applied biochemistry, 40(1): S. 47-55, 2004.
• Weber, Christian and Pohl, Sebastian and Pörtner, Ralf and Wallrapp, Christine and Kassem, Moustapha and Geigle, Peter and Czermak, Peter: Expansion and Harvesting of hMSC-TERT The open biomedical engineering journal, 1: S. 38-46, 2007.
• Weber, Christian and Freimark, Denise and Pörtner, Ralf and Pino-Grace, Pablo and Pohl, Sebastian and Wallrapp, Christine and Geigle, Peter and Czermak, Peter: Expansion of human mesenchymal stem cells in a fixed-bed bioreactor system based on non-porous glass carrier - Part B: Modeling and scale-up of the system The International journal of artificial organs, 33(11): S. 782--795, 2010.
• Weber, Christian and Freimark, Denise and Pörtner, Ralf and Pino-Grace, Pablo and Pohl, Sebastian and Wallrapp, Christine and Geigle, Peter and Czermak, Peter: Expansion of human mesenchymal stem cells in a fixed-bed bioreactor system based on non-porous glass carrier--part A: inoculation, cultivation, and cell harvest procedures The International journal of artificial organs, 33(8): S. 512-525, 2010.
• Nehring, D and R. Gonzales and P. Czermak and R. Pörtner:: Experimental and modeling study of a membrane filtration process using ceramic membranes to increase retroviral pseudotype vector titer Journal of Membrane Science, 2004(237): S. 25--38, 2004.
• Nehring, Dirk and Gonzalez, Roberto and Pörtner, Ralf and Czermak, Peter: Experimental and modelling study of different process modes for retroviral production in a fixed bed reactor Journal of biotechnology, 122(2): S. 239-253, 2006.
• Fassnacht, D and Pörtner, R: Experimental and theoretical considerations on oxygen supply for animal cell growth in fixed-bed reactors Journal of biotechnology, 72(3): S. 169-184, 1999.
• Frahm, B and Hass, V C and Lane, P and Munack, A and Märkl, H and Pörtner, R: Fed-Batch-Kultivierung tierischer Zellen - Eine Herausforderung zur adaptiven, modellbasierten Steuerung Chemie Ingenieur Technik, 75(4): S. 457--460, 2003.
• Pörtner, R and Lüdemann, I and Reher, K and Neumaier, M and Märkl, H: Fixed-bed dialysis culture of a transfectoma cell line producing chimeric Fab-fragments with "nutrient-split"-feeding strategy Biotechnology Techniques, 12(7): S. 501--505, 1998.
• Becker, Thomas and Hitzmann, Bernd and Muffler, K and Pörtner, Ralf and Reardon, Kenneth F and Stahl, Frank and Ulber, Roland: Future aspects of bioprocess monitoring Advances in biochemical engineering/biotechnology, 105: S. 249-293, 2007.
• Ong, C P and Pörtner, R and Märkl, H and Yamazaki, Y and Yasuda, K and Matsumura, M: High density cultivation of hybridoma in charged porous carriers Journal of biotechnology, 34(3): S. 259-268, 1994.
• Pörtner, R and Schilling, A and Lüdemann, I and Märkl, H: High density fed-batch cultures for hybridoma cells performed with the aid of a kinetic model Bioprocess Engineering, 15(3): S. 117--124, 1996.
• Hißnauer, Tim-Nicolas and Baranowsky, Anke and Pestka, Jan M and Streichert, Thomas and Wiegandt, K and Goepfert, C and Beil, Frank Timo and Albers, Joachim and Schulze, Jochen and Ueblacker, Peter and Petersen, Jan-Philipp and Schinke, Thorsten and Meenen, N M and Pörtner, R and Amling, Michael: Identification of molecular markers for articular cartilage Osteoarthritis and cartilage, 18(12): S. 1630--1638, 2010.
• Frahm, B and Lane, P and Märkl, H and Pörtner, R: Improvement of a mammalian cell culture process by adaptive, model-based dialysis fed-batch cultivation and suppression of apoptosis Bioprocess and biosystems engineering, 26(1): S. 1--10, 2003.
• Lüdemann, I and Pörtner, R and Schaefer, C and Schick, K and Sr\'amkova, K and Reher, K and Neumaier, M and Fran\vek, F and Märkl, H: Improvement of the culture stability of non-anchorage-dependent animal cells grown in serum-free media through immobilization Cytotechnology, 19(2): S. 111-124, 1995.
• Schwabe, J O and Pörtner, R and Märkl, H: Improving an on-line feeding strategy for fed-batch cultures of hybridoma cells by dialysis and `Nutrient-Split'-feeding Bioprocess Engineering, 20(6): S. 475-484, 1999.
• Wiegandt, Katharina and Goepfert, Christiane and Pörtner, Ralf: Improving in vitro generated cartilage-carrier-constructs by optimizing growth factor combination The open biomedical engineering journal, 1: S. 85-90, 2007.
• Hussein, M A and Esterl, S and Pörtner, R and Wiegandt, K and Becker, T: Impulse and mass transport in a cartilage bioreactor using the lattice Boltzmann method International Journal of Computational Fluid Dynamics, 22(5): S. 341--350, 2008.
• Meyer, Stefanie and Peters, Nils and Mann, Tobias and Wolber, Rainer and Pörtner, Ralf and Nierle, Jens: In vitro efficacy and release study with anti-inflammatory drugs incorporated in adhesive transdermal drug delivery systems Journal of pharmaceutical sciences, 103(4): S. 1142-1148, 2014.
• Wiegandt, Katharina and Goepfert, Christiane and Richter, Teresa and Fritsch, Daniel and Janßen, Rolf and Pörtner, Ralf: In vitro generation of cartilage-carrier-constructs on hydroxylapatite ceramics with different surface structures The open biomedical engineering journal, 2: S. 64-70, 2008.
• Fassnacht, D and Rössing, S and Singh, R P and Al-Rubeai, M and Pörtner, R: Influence of bcl-2 on antibody productivity in high cell density perfusion cultures of hybridoma Cytotechnology, 30(1-3): S. 95-106, 1999.
• Fassnacht, D and Ghaussy, N and Rössing, S and Pörtner, R: Influence of non-essential amino acids on apoptotic and necrotic death of mouse hybridoma cells in batch cultures Biotechnology Letters, 19(1): S. 35--38, 1997.
• Hass, V C and Kuhnen, F and Schoop, K.-M and Pörtner, R: Integration von computerbasiertem Lernen und Training in die bioverfahrenstechnische Ausbildung Chemie Ingenieur Technik, 77(8): S. 1250-1251, 2005.
• Wiegandt, K and Goepfert, C and Pörtner, R and Janssen, R: Interaction of cartilage and ceramic matrix Advances in biochemical engineering/biotechnology, 126: S. 297--315, 2012.
• Pörtner, R and Hass, V C: Interaktive virtuelle Lernumgebung für die Biotechnologie (eLearnBioTec) Chemie Ingenieur Technik, 77(8): S. 1256, 2005.
• Weiss, K D Salzig M D Mühlebach K Cichutek R Pörtner and Czermak, P: Key parameters of measles virus production for oncolytic virotherapy American Journal of Biochemistry and Biotechnology, 2012(8 (2)): S. 81-98, 2012.
• Pörtner, R and Rössing, S and Koop, M and Lüdemann, I: Kinetic studies on hybridoma cells immobilized in fixed bed reactors Biotechnology and Bioengineering, 55(3): S. 535-541, 1997.
• Meenen, N M and Ueblacker, P and Pörtner, R and Göpfert, C and Nagel-Heyer, S and Petersen, J P and Adamietz, P: Knorpel aus dem Labor - eine Sackgasse? Arthroskopie, 18(3): S. 245-252, 2005.
• Petersen, J P and Ueblacker, P and Goepfert, C and Adamietz, P and Baumbach, K and Stork, A and Rueger, J M and Poertner, R and Amling, M and Meenen, N M: Long term results after implantation of tissue engineered cartilage for the treatment of osteochondral lesions in a minipig model Journal of materials science. Materials in medicine, 19(5): S. 2029-2038, 2008.
• Fassnacht, D and Rossing, S and Stange, J and Portner, R: Long-term cultivation of immortalised mouse hepatocytes in a high cell density fixed bed reactor Biotechnology Techniques, 12(1): S. 25-30, 1998.
• Kern, Simon and Platas, Oscar B and Schaletzky, Martin and Sandig, Volker and Frahm, Björn and Pörtner, Ralf: Model-based design of the first steps of a seed train for cell culture processes BMC Proceedings, 7(Suppl 6): S. P11, 2013.
• Pörtner, R and Schäfer, T: Modelling hybridoma cell growth and metabolism-a comparison of selected models and data Journal of biotechnology, 49(1-3): S. 119-135, 1996.
• Hussein, M A and Esterl, S and Pörtner, R and Wiegandt, K and Becker, T: On the lattice Boltzmann method simulation of a two-phase flow bioreactor for artificially grown cartilage cells Journal of biomechanics, 41(16): S. 3455-3461, 2008.
• Rausch, Mareike Katharina: Optimierung der Proteinexpression in Insektenzellen und Scale-up in den Wave-Bioreaktor. Promotion/Dissertation, TUHH, Institut für Bioprozess- und Biosystemtechnik, , 2011.
• Frahm, B and Pörtner, R and Lane, P and Munack, A: Optimierung und Steuerung von Zellkultur-Fed-Batch-Prozessen mittels einer Kollokationsmethode Chemie Ingenieur Technik, 77(4): S. 429-435, 2005.
• Bohmann, A and Pörtner, R and Märkl, H: Performance of a membrane-dialysis bioreactor with a radial-flow fixed bed for the cultivation of a hybridoma cell line Applied microbiology and biotechnology, 43(5): S. 772-780, 1995.
• Nehring, Dirk and Adamietz, Peter and Meenen, Norbert M and Pörtner, Ralf: Perfusion cultures and modelling of oxygen uptake with three-dimensional chondrocyte pellets Biotechnology Techniques, 13(10): S. 701-706, 1999.
• Pörtner, Ralf and M. Matsumura and T. Hatae and K. Murakami and H. Kataoka: Perfusion-microcarrier cultivation of rCHO cells in serum free medium for production of human renin Bioprocess Engineering, 1991(7): S. 63-69, 1991.
• Hass, Volker C and Pörtner, Ralf: Praxis der Bioprozesstechnik: Mit virtuellem Praktikum. Spektrum Akad. Verl, Heidelberg, , 2009
• Hass, Volker C and Pörtner, Ralf: Praxis der Bioprozesstechnik: Mit virtuellem Praktikum. Spektrum Akad. Verl, Heidelberg, , 2, 2011
• Druzinec, Damir and Weiss, Katja and Elseberg, Christiane and Salzig, Denise and Kraume, Matthias and Pörtner, Ralf and Czermak, Peter: Process analytical technology (PAT) in insect and mammalian cell culture processes: dielectric spectroscopy and focused beam reflectance measurement (FBRM) Methods in molecular biology (Clifton, N.J.), 1104: S. 313--341, 2014.
• Weber, C and Pohl, S and Poertner, R and Pino-Grace, Pablo and Freimark, D and Wallrapp, C and Geigle, P and Czermak, P: Production process for stem cell based therapeutic implants: expansion of the production cell line and cultivation of encapsulated cells Advances in biochemical engineering/biotechnology, 123: S. 143--162, 2010.
• Pörtner, Ralf: Reaktionstechnik der Kultur tierischer Zellen. Shaker, Aachen, , Als Ms. gedr., 1998
• Heyland, Jan and Wiegandt, Katharina and Goepfert, Christiane and Nagel-Heyer, Stefanie and Ilinich, Eduard and Schumacher, Udo and Pörtner, Ralf: Redifferentiation of chondrocytes and cartilage formation under intermittent hydrostatic pressure Biotechnology letters, 28(20): S. 1641-1648, 2006.
• Fussenegger, M and Fassnacht, D and Schwartz, R and Zanghi, J A and Graf, M and Bailey, J E and Pörtner, R: Regulated overexpression of the survival factor bcl-2 in CHO cells increases viable cell density in batch culture and decreases DNA release in extended fixed-bed cultivation Cytotechnology, 32(1): S. 45-61, 2000.
• Fassnacht, Dieter and I. Reimann, R Pörtner und H Märkl: Scale-up von Festbettreaktoren zur Kultivierung tierischer Zellen Chemie Ingenieur Technik, 2001(73): S. 1075-1079, 2001.
• Hernandez Rodriguez, Tanja and Pörtner, Ralf and Frahm, Björn: Seed train optimization for suspension cell culture BMC Proceedings, 7(Suppl 6): S. P9, 2013.
• Cantürk, Cumhur and Baade, Ulrike and Salazar, Ramona and Storm, Niels and Pörtner, Ralf and Höppner, Wolfgang: Sequence analysis of CYP21A1P in a German population to aid in the molecular biological diagnosis of congenital adrenal hyperplasia Clinical chemistry, 57(3): S. 511-517, 2011.
• Pörtner, R and Goepfert, C and Wiegandt, K and Janssen, R and Ilinich, E and Paetzold, H and Eisenbarth, E and Morlock, M: Technical strategies to improve tissue engineering of cartilage-carrier-constructs Advances in biochemical engineering/biotechnology, 112: S. 145-181, 2009.
• Pörtner, R and Meenen, N M: Technische Aspekte in regenerativer Medizin und Tissue Engineering von hyalinem Gelenkknorpel Handchirurgie, Mikrochirurgie, plastische Chirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft f\ü\r Handchirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft f\ü\r Mikrochirurgie der Peripheren Nerven und Gef\äße : Organ der Vereinigung der Deutschen Plastischen Chirurgen, 42(6): S. 329-336, 2010.
• Bohmann, A and Pörtner, R and Schmieding, J and Kasche, V and Märkl, H: The membrane dialysis bioreactor with integrated radial-flow fixed bed - a new approach for continuous cultivation of animal cells Cytotechnology, 9(1-3): S. 51-57, 1992.
• Kasper, Cornelia and Witte, Frank and Pörtner, Ralf: Tissue Engineering III: Cell - Surface Interactions for Tissue Culture, Jg. 126 von Advances in Biochemical Engineering Biotechnology. Springer Berlin Heidelberg, Berlin Heidelberg, , 2012
• Freimark, D and Pino-Grace, P and Pohl, S and Weber, C and Wallrapp, C and Geigle, P and Pörtner, R and Czermak, P: Use of Encapsulated Stem Cells to Overcome the Bottleneck of Cell Availability for Cell Therapy Approaches Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie, 37(2): S. 66-73, 2010.