Dr. rer. nat. Anna Krüger


 

 

Research topics and industrial cooperation

 

  • Enzymatic oxidation systems for food and technical applications
  • High-throughput screening of extremophilic microorganisms for oxidative enzymes
  • Fermentation strategies with E. coli, B. subtilis or P. pastoris as expression hosts for the production of oxidative enzymes
  • Synthetic biology as means for enzyme optimization
  • Production of enzyme congeners by incorporation of non-canonical amino acids
  • High-throughput characterization of extremozymes and enzyme congeners

 

Curriculum Vitae

2016- Senior Scientific Officer at the Institute of Technical Microbiology, Hamburg University of Technology, Hamburg, Germany
2011-2016 Project leader and lecturer at the Institute of Technical Microbiology, Hamburg University of Technology, Hamburg, Germany
2007-2011 Doctoral thesis at the Institute of Technical Microbiology, Hamburg University of Technology, Hamburg, Germany. Title: “Cold-active microbial oxidoreductases from Arctic sea ice and the deep sea”
2007

Diploma thesis at the Department of Plant Sciences, University of Cambridge, Cambridge, UK. Title: “Tetrapyrrole synthesis and the regulation of nuclear gene expression”

2005

Student research project at the Department of Plant Sciences, University of Cambridge, Cambridge, UK. Title: “Analysis of the tetrapyrrole pathway in Arabidopsis thaliana

2002-2007 Studies in biotechnology at Carolo-Wilhelmina Technical University of Braunschweig, Braunschweig, Germany
1983 Born in Hannover, Germany

 

Memberships

2008- VAAM – Vereinigung für Allgemeine und Angewandte Mikrobiologie
2009-2017btS – Biotechnologische Studenteninitiative e.V.
2014-2015ISE – International Society for Extremophiles

 

Publications

2020

Suleiman M, Krüger A and Antranikian G (2020) Biomass‑degrading glycoside hydrolases of archaeal origin. Biotechnology for Biofuels 13, 153. doi.org/10.1186/s13068-020-01792-y

Krüger A, Schäfers C, Busch B and Antranikian G (2020) Digitalization in microbiology – Paving the path to sustainable circular bioeconomy. New Biotechnology, Volume 59, pp. 88-96; doi.org/10.1016/j.nbt.2020.06.004

2018

Suleiman M, Schröder C, Klippel B, Schäfers C, Krüger A, Antranikian G (2018) Extremely thermoactive archaeal endoglucanase from a shallow marine hydrothermal vent from Vulcano Island. Applied Microbiology and Biotechnology, doi.org/10.1007/s00253-018-9542-z

Krüger A, Schäfers C, Schröder C, Antranikian G (2018) Towards a sustainable biobased industry – Highlighting the impact of extremophiles, New Biotechnology; Volume 40, Part A, pp. 144-153; doi: doi.org/10.1016/j.nbt.2017.05.002

2016

Elleuche S, Esselun P, Grote R, Klippel B, Krüger A, Liese A, Ostrop M, Schäfers C, Schröder C, Antranikian G (2016) 8th International Congress on Biocatalysis (Biocat2016), Hamburg, Germany, 28 August–1 September, 2016 (Meeting Report). Catalysts 2016, 6, 160; doi:10.3390/catal6100160

Krüger A, Elleuche S, Sahm K, Antranikian G (2016) Robust biocatalysts - routes to new diversity. In: Hilterhaus L, Liese A, Kettling U, Antranikian G (Eds.) Applied Biocatalysis: From Fundamental Science to Industrial Applications. Wiley-VCH Verlag GmbH; pp. 31-51

2008

Rotthues A, Kappler J, Lichtfuß A, Kloos, DU, Stahl, DJ, Hehl, R (2008) Post-harvest regulated gene expression and splicing efficiency in storage roots of sugar beet (Beta vulgaris L.). Planta 227, 1321-1332

 

Patents

2019

Neue Amylasen. EP3464577A1

2017

Verbesserte Waschleistung durch eine alpha-Amylase aus Bacillus cereus. DE102016201637A1

Improved wash performance using an alpha-amylase from Bacillus cereus. DE102016201643A1

Improved washing performance using an alpha-amylase from Bacillus cereus. WO2017133973A1

Verbesserte Waschleistung durch eine alpha-Amylase aus Bacillus cereus. WO2017133974A1

Neue Amylasen. WO2017211678A1

 

Conference contributions (posters and oral presentations)

2020

27. *Krüger A, Meyer-Heydecke N, Suleiman M, Schäfers C, Antranikian G (2020) Discovering the Earth’s extremes – Bioprospecting for extremozymes in volcano-associated habitats in Costa Rica. Programme of the 6th Joint Conference of DGHM & VAAM 2020, Leipzig, Germany, p. 58 (oral presentation)

2019

26. *Krüger A, Suleiman M, Schröder C, Klippel B, Schäfers C, Antranikian G (2019) Identification of Vul_Cel5A – the most thermoactive endoglucanase described to date. 7th Workshop of the Research Center Integrated Biotechnology and Process Engineering, Hamburg, Germany, Yearbook 2019, p. 75f.

25. *Meyer-Heydecke N, Krüger A, Peters A, Antranikian G (2019) Activity-based screening for the identification of novel α-amylases for industrial processes and daily applications. 7th Workshop of the Research Center Integrated Biotechnology and Process Engineering, Hamburg, Germany, Yearbook 2019, p. 97f.

24. Waluga T, Kraus Y, Krüger A, *Johannsen J (2019) An innovative method for the characterization of multi-substrate enzyme reactions. AIChE Annual Meeting, Orlando, USA

23. *Heerenklage J, Rechtenbach D, Atamaniuk I, Alassali A, Graage A-C, Pasternak P, Krüger A, Muntoni A, Isipato M, Kuchta K (2019) Investigation of standardised and adapted inocula for biomethane potential tests. 17th International Waste Management and Landfill Symposium, Sardinia, Italy (oral presentation)

22. *Heerenklage J, Rechtenbach D, Atamaniuk I, Alassali A, Zimmermann J, von Borstel N, Müller F, Tiemann CM, Krüger A, Graage A-C, Kuchta K (2019) Lab tests with a mini-bioreactor test system (all-in-one) for the development of standardized and storable inocula for BMP tests. IV. Conference on Monitoring & Process Control of Anaerobic Digestion Plants, Leipzig, Germany (oral presentation)

21. Suleiman M, *Krüger A, Schröder C, Klippel B, Schäfers C, Antranikian G (2019) Extremely thermoactive archaeal endoglucanase from a shallow marine hydrothermal vent from Vulcano Island. Tagungsband zur VAAM-Jahrestagung 2019, Mainz, Germany, p. 109f. (oral presentation)

2018

20. *Krüger A, Meyer-Heydecke N, Suleiman M, Peters A, Schäfers C, Antranikian G (2018) Bioprospecting for novel extremozymes with industrial potential. 6th Workshop of the Research Center Integrated Biotechnology and Process Engineering, Hamburg, Germany, Yearbook 2018, p. 99f. (oral presentation)

19. *Meyer-Heydecke N, *Krüger A, Peters A, Schäfers C, Antranikian G (2018) Identification of novel ᴅ-carbamoylases for the production of optically pure amino acids. 9th International Congress on Biocatalysis, biocat2016, eBook of Abstracts, p. 81

18. *Meyer-Heydecke N, Krüger A, Peters A, Antranikian G (2018) A novel α-amylase with remarkable characteristics for biotechnological applications. Tagungsband zur VAAM-Jahrestagung 2018, Wolfsburg, Germany, p. 67

2017

17. Krüger A, *Piascheck H, Nölle V, Odenthal U, Villain L, Beutel S, Scheper T, Antranikian G (2017) Innovative downstream processing techniques for the production of recombinant cytokines based on membrane adsorbers. 5th Workshop of the Research Center Integrated Biotechnology and Process Engineering, Yearbook 2017, p. 71f.

16. *Krüger A, Piascheck H, Nölle V, Odenthal U, Villain L, Beutel S, Scheper T, Antranikian G (2017) Innovative downstream processing techniques for the production of recombinant cytokines based on membrane adsorbers. Tagungsband zur 5. Gemeinsamen Konferenz von DGHM und VAAM, Würzburg, Germany, p. 336

2016

15. *Krüger A, Welsch N, Piascheck H, Beyer S, Kabisch J, Popper L, Antranikian G, Schweder T (2016) Production of sulfhydryl oxidase from Saccharomyces cerevisiae with Bacillus subtilis expression strains. 8th International Congress on Biocatalysis, biocat2016, eBook of Abstracts, Ed: Antranikian G. TuTech Innovation GmbH, TuTech Verlag, Hamburg. ISBN: 978-3-946094-07-4

14. *Meyer N, Krüger A, Peters A, Antranikian G (2016) Activity-based screening for the identification of novel α-amylases for industrial processes and daily applications. 8th International Congress on Biocatalysis, biocat2016, eBook of Abstracts, Ed: Antranikian G. TuTech Innovation GmbH, TuTech Verlag, Hamburg. ISBN: 978-3-946094-07-4

2014

13. *Krüger A, Hoesl MG, Peters A, Budisa N, Antranikian G (2014) Enhancing industrially relevant lipolytic enzymes by genetic code engineering. 3rd Workshop of the Research Center Integrated Biotechnology and Process Engineering, Hamburg, Germany, Yearbook 2014, p. 118

12. *Krüger A, Hoesl MG, Peters A, Budisa N, Antranikian G (2014) Synthetic biology as means for enzyme optimization: improving Thermoanaerobacter tengcongensis esterase by incorporation of non-canonical amino acids. Tagungsband zur 4. Gemeinsamen Konferenz von DGHM und VAAM, Dresden, Germany, p. 82 (oral presentation)

11. *Krüger A, Antranikian G (2014) Cold-active microbial oxidoreductases from Arctic sea ice and the deep sea. 10th International Congress on Extremophiles, St. Petersburg, Russia, Book of Abstracts, p. 287

10. *Krüger A, Hoesl MG, Peters A, Budisa N, Antranikian G (2014) Improving industrially relevant enzymes by incorporation of non-canonical amino acids. 7th International Congress on Biocatalysis, Hamburg, Germany, Book of Abstracts, p. 140

9. *Chatziefthimiou SD, Pinotsis N, Krüger A, Marrakchi H, Royter M, Daffe M, Antranikian G, Wilmanns M (2014) The structure of a thermoactive lipase from Thermoanaerobacter thermohydrosulfuricus: snapshots of the lipase mechanism. 7th International Congress on Biocatalysis, Hamburg, Germany, Book of Abstracts, p. 106 (oral presentation)

2013

8. *Krüger A, Hoesl MG, Chatziefthimiou SD, Wilmanns M, Budisa N, Antranikian G (2013) P29 Lipase congeners with improved catalytic activity and substrate access. BIOKATALYSE2021 Clusterkonferenz 2013, Hamburg, Germany

7. *Krüger A, Klippel B, Antranikian G (2013) PZ: Zentrales Technologie- und Serviceprojekt für Screening, Fermentation und Proteinreinigung. BIOKATALYSE2021 Clusterkonferenz 2013, Hamburg, Germany

2012

6. *Krüger A, Antranikian G (2012) Characterization of cold-active α-glycerophosphate oxidase from Carnobacterium sp. 33-1. 6th International Congress on Biocatalysis, Hamburg, Germany, Book of Abstracts, p. 133

5. *Boysen H, Krüger A, Piascheck H, Halbsguth T, Antranikian G (2012) Produktion einer Sulfhydryl-Oxidase mit Bacillus subtilis. Tagungsband zur 9. Hamburger Studententagung „Innovative Medizin- und Biotechnologie aus Hamburg", Hamburg, Germany, p. 38

4. *Hoesl MG, Krüger A, Winkelmann N, Elleuche S, Wiltschi B, Antranikian G, Budisa N (2012) Enhancing industrially relevant enzymes with an expanded genetic code. European Federation of Biotechnology, 1st Congress on Applied Synthetic Biology in Europe 2012, Barcelona, Spain

2011

3. *Krüger A, Antranikian G (2011) Characterization of α-glycerophosphate oxidase from Carnobacterium sp. 33-1. 1st Workshop of the Research Center Integrated Biotechnology and Process Engineering, Hamburg, Germany, p. III

2. *Krüger A, Antranikian G (2011) Oxidoreductases from extremophilic microorganisms isolated from Spitsbergen and the deep sea. Tagungsband zur VAAM-Jahrestagung 2011, Karlsruhe, Germany, p. 168

2009

1. *Lichtfuß A, Antranikian G (2009) Glycerol degradation by psychrophilic bacteria. Tagungsband zur VAAM-Jahrestagung 2009, Bochum, Germany, p. 200