Dr. rer. nat. Anna Krüger

 

 

Research topics and industrial cooperation

 

  • Enzymatic oxidation systems for food and technical applications
  • High-throughput screening of extremophilic microorganisms for oxidative enzymes
  • Fermentation strategies with E. coli, B. subtilis or P. pastoris as expression hosts for the production of oxidative enzymes
  • Synthetic biology as means for enzyme optimization
  • Production of enzyme congeners by incorporation of non-canonical amino acids
  • High-throughput characterization of extremozymes and enzyme congeners

 

Curriculum vitae

2016- Senior Scientific Officer at the Institute of Technical Microbiology, Hamburg University of Technology, Hamburg, Germany
2011-2016 Project leader and lecturer at the Institute of Technical Microbiology, Hamburg University of Technology, Hamburg, Germany
2007-2011 Doctoral thesis at the Institute of Technical Microbiology, Hamburg University of Technology, Hamburg, Germany. Title: “Cold-active microbial oxidoreductases from Arctic sea ice and the deep sea”
2007

Diploma thesis at the Department of Plant Sciences, University of Cambridge, Cambridge, UK. Title: “Tetrapyrrole synthesis and the regulation of nuclear gene expression”

2005

Student research project at the Department of Plant Sciences, University of Cambridge, Cambridge, UK. Title: “Analysis of the tetrapyrrole pathway in Arabidopsis thaliana

2002-2007 Studies in biotechnology at Carolo-Wilhelmina Technical University of Braunschweig, Braunschweig, Germany
1983 Born in Hannover, Germany

 

Memberships

2008- VAAM – Vereinigung für Allgemeine und Angewandte Mikrobiologie
2009-2017btS – Biotechnologische Studenteninitiative e.V.
2014-2015ISE – International Society for Extremophiles

 

Publications

2018

Suleiman M, Schröder C, Klippel B, Schäfers C, Krüger A, Antranikian G (2018) Extremely thermoactive archaeal endoglucanase from a shallow marine hydrothermal vent from Vulcano Island. Applied Microbiology and Biotechnology, doi.org/10.1007/s00253-018-9542-z

Krüger A, Schäfers C, Schröder C, Antranikian G (2018) Towards a sustainable biobased industry – Highlighting the impact of extremophiles, New Biotechnology; Volume 40, Part A, pp. 144-153; doi: doi.org/10.1016/j.nbt.2017.05.002

2016

Elleuche S, Esselun P, Grote R, Klippel B, Krüger A, Liese A, Ostrop M, Schäfers C, Schröder C, Antranikian G (2016) 8th International Congress on Biocatalysis (Biocat2016), Hamburg, Germany, 28 August–1 September, 2016 (Meeting Report). Catalysts 2016, 6, 160; doi:10.3390/catal6100160

Krüger A, Elleuche S, Sahm K, Antranikian G (2016) Robust biocatalysts - routes to new diversity. In: Hilterhaus L, Liese A, Kettling U, Antranikian G (Eds.) Applied Biocatalysis: From Fundamental Science to Industrial Applications. Wiley-VCH Verlag GmbH; pp. 31-51

2008 

Rotthues A, Kappler J, Lichtfuß A, Kloos, DU, Stahl, DJ, Hehl, R (2008) Post-harvest regulated gene expression and splicing efficiency in storage roots of sugar beet (Beta vulgaris L.). Planta 227, 1321-1332

 

Patents

Neue Amylasen. WO2017211678A1 (2017) / Neue Amylasen. EP3464577A1 (2019)

Verbesserte Waschleistung durch eine alpha-Amylase aus Bacillus cereus. DE102016201637A1 / Improved wash performance using an alpha-amylase from Bacillus cereus. DE102016201643A1 (2017)

Improved washing performance using an alpha-amylase from Bacillus cereus. WO2017133973A1 / Verbesserte Waschleistung durch eine alpha-Amylase aus Bacillus cereus. WO2017133974A1 (2017)

 

Conference contributions (posters and oral presentations)

23. *Waluga T, Kraus Y, Krüger A, Johannsen J (2019) An innovative method for the characterization of multi-substrate enzyme reactions. AIChE Annual Meeting, Orlando, USA

22. *Heerenklage J, Rechtenbach D, Atamaniuk I, Alassali A, Zimmermann J, von Borstel N, Müller F, Tiemann CM, Krüger A, Graage A-C, Kuchta K (2019) Lab tests with a mini-bioreactor test system (all-in-one) for the development of standardized and storable inocula for BMP tests. IV. Conference on Monitoring & Process Control of Anaerobic Digestion Plants, Leipzig, Germany (oral presentation)

21. Suleiman M, *Krüger A, Schröder C, Klippel B, Schäfers C, Antranikian G (2019) Extremely thermoactive archaeal endoglucanase from a shallow marine hydrothermal vent from Vulcano Island. Tagungsband zur VAAM-Jahrestagung 2019, Mainz, Germany, p. 109f. (oral presentation)

20. *Krüger A, Meyer-Heydecke N, Suleiman M, Peters A, Schäfers C, Antranikian G (2018) Bioprospecting for novel extremozymes with industrial potential. 6th Workshop of the Research Center Integrated Biotechnology and Process Engineering, Hamburg, Germany, Yearbook 2018, p. 99f. (oral presentation)

19. *Meyer-Heydecke N, *Krüger A, Peters A, Schäfers C, Antranikian G (2018) Identification of novel ᴅ-carbamoylases for the production of optically pure amino acids. 9th International Congress on Biocatalysis, biocat2016, eBook of Abstracts, p. 81

18. *Meyer-Heydecke N, Krüger A, Peters A, Antranikian G (2018) A novel α-amylase with remarkable characteristics for biotechnological applications. Tagungsband zur VAAM-Jahrestagung 2018, Wolfsburg, Germany, p. 67

17. Krüger A, *Piascheck H, Nölle V, Odenthal U, Villain L, Beutel S, Scheper T, Antranikian G (2017) Innovative downstream processing techniques for the production of recombinant cytokines based on membrane adsorbers. 5th Workshop of the Research Center Integrated Biotechnology and Process Engineering, Yearbook 2017, p. 71f.

16. *Krüger A, Piascheck H, Nölle V, Odenthal U, Villain L, Beutel S, Scheper T, Antranikian G (2017) Innovative downstream processing techniques for the production of recombinant cytokines based on membrane adsorbers. Tagungsband zur 5. Gemeinsamen Konferenz von DGHM und VAAM, Würzburg, Germany, p. 336

15. *Krüger A, Welsch N, Piascheck H, Beyer S, Kabisch J, Popper L, Antranikian G, Schweder T (2016) Production of sulfhydryl oxidase from Saccharomyces cerevisiae with Bacillus subtilis expression strains. 8th International Congress on Biocatalysis, biocat2016, eBook of Abstracts, Ed: Antranikian G. TuTech Innovation GmbH, TuTech Verlag, Hamburg. ISBN: 978-3-946094-07-4

14. *Meyer N, Krüger A, Peters A, Antranikian G (2016) Activity-based screening for the identification of novel α-amylases for industrial processes and daily applications. 8th International Congress on Biocatalysis, biocat2016, eBook of Abstracts, Ed: Antranikian G. TuTech Innovation GmbH, TuTech Verlag, Hamburg. ISBN: 978-3-946094-07-4

13. *Krüger A, Hoesl MG, Peters A, Budisa N, Antranikian G (2014) Enhancing industrially relevant lipolytic enzymes by genetic code engineering. 3rd Workshop of the Research Center Integrated Biotechnology and Process Engineering, Hamburg, Germany, Yearbook 2014, p. 118

12. *Krüger A, Hoesl MG, Peters A, Budisa N, Antranikian G (2014) Synthetic biology as means for enzyme optimization: improving Thermoanaerobacter tengcongensis esterase by incorporation of non-canonical amino acids. Tagungsband zur 4. Gemeinsamen Konferenz von DGHM und VAAM, Dresden, Germany, p. 82 (oral presentation)

11. *Krüger A, Antranikian G (2014) Cold-active microbial oxidoreductases from Arctic sea ice and the deep sea. 10th International Congress on Extremophiles, St. Petersburg, Russia, Book of Abstracts, p. 287

10. *Krüger A, Hoesl MG, Peters A, Budisa N, Antranikian G (2014) Improving industrially relevant enzymes by incorporation of non-canonical amino acids. 7th International Congress on Biocatalysis, Hamburg, Germany, Book of Abstracts, p. 140

9. *Chatziefthimiou SD, Pinotsis N, Krüger A, Marrakchi H, Royter M, Daffe M, Antranikian G, Wilmanns M (2014) The structure of a thermoactive lipase from Thermoanaerobacter thermohydrosulfuricus: snapshots of the lipase mechanism. 7th International Congress on Biocatalysis, Hamburg, Germany, Book of Abstracts, p. 106 (oral presentation)

8. *Krüger A, Hoesl MG, Chatziefthimiou SD, Wilmanns M, Budisa N, Antranikian G (2013) P29 Lipase congeners with improved catalytic activity and substrate access. BIOKATALYSE2021 Clusterkonferenz 2013, Hamburg, Germany

7. *Krüger A, Klippel B, Antranikian G (2013) PZ: Zentrales Technologie- und Serviceprojekt für Screening, Fermentation und Proteinreinigung. BIOKATALYSE2021 Clusterkonferenz 2013, Hamburg, Germany

6. *Krüger A, Antranikian G (2012) Characterization of cold-active α-glycerophosphate oxidase from Carnobacterium sp. 33-1. 6th International Congress on Biocatalysis, Hamburg, Germany, Book of Abstracts, p. 133

5. *Boysen H, Krüger A, Piascheck H, Halbsguth T, Antranikian G (2012) Produktion einer Sulfhydryl-Oxidase mit Bacillus subtilis. Tagungsband zur 9. Hamburger Studententagung „Innovative Medizin- und Biotechnologie aus Hamburg", Hamburg, Germany, p. 38

4. *Hoesl MG, Krüger A, Winkelmann N, Elleuche S, Wiltschi B, Antranikian G, Budisa N (2012) Enhancing industrially relevant enzymes with an expanded genetic code. European Federation of Biotechnology, 1st Congress on Applied Synthetic Biology in Europe 2012, Barcelona, Spain

3. *Krüger A, Antranikian G (2011) Characterization of α-glycerophosphate oxidase from Carnobacterium sp. 33-1. 1st Workshop of the Research Center Integrated Biotechnology and Process Engineering, Hamburg, Germany, p. III

2. *Krüger A, Antranikian G (2011) Oxidoreductases from extremophilic microorganisms isolated from Spitsbergen and the deep sea. Tagungsband zur VAAM-Jahrestagung 2011, Karlsruhe, Germany, p. 168

1. *Lichtfuß A, Antranikian G (2009) Glycerol degradation by psychrophilic bacteria. Tagungsband zur VAAM-Jahrestagung 2009, Bochum, Germany, p. 200