Forschungsbericht 2005

2-09 Bioprozess- und Bioverfahrenstechnik

Bioprozeß- und Bioverfahrenstechnik

Forschungsprojekte

• E.2-09.026 Nachhaltige Produktion hochwertiger Naturfasern
• E.2-09.027 Scale-up eines Verfahrens zur hochdichten Kultivierung rekombinanter Escherichia coli
• E.2-09.029 Novel bioreduction by hyperthermophilic microorganisms for the natural, specific and on-line production of fine chemicals
• E.2-09.032 Ingenieurtechnische Aspekte bei der Herstellung künstlicher Gelenkknorpelimplantate
• E.2-09.034 Interaktive virtuelle Lernumgebung für die Biotechnologie (e-LearnBioTec)

Kooperationen

• Aventis Pharma, Frankfurt/M
• Bioengineering AG, Wald, Schweiz
• Biokraftwerke Fürstenwalde GmbH
• Biomet Merck BioMaterials, Darmstadt
• CellConcept, Umkirch
• Deutsche Hefewerke, Hamburg
• Kurt-Schwabe-Institut, Meinsberg
• Lonza Biologics, Slough, UK
• Prof. Dr. A. Munack, Forschungsanstalt für Landwirtschaft, Braunschweig
• Prof. Dr. M. Al-Rubeai, University of Birmingham, UK
• Prof. Dr. M. Matsumura, University of Tsukuba, Japan
• Prof. Dr. M. Rossi, University of Naples, Neapel, Italien
• Prof. Dr. R. Ladenstein, Karolinska Institute, Stockholm, Schweden
• Prof. Dr. T. Scheper, Universität Hannover
• Prof. Dr. W. de Vos, Wageningen Agricultural University, Wageningen, Niederlande
• Prof. Dr.-Ing. V. Hass, HS Bremen
• Rauschert Verfahrenstechnik GmbH, Steinwiesen
• Universitätsklinikum Eppendorf, Hamburg
• ZellWerk GmbH, Eichstädt
• meredos GmbH, Bovenden

Publikationen

Veröffentlichungen

• 2-09.001V
  Müller, R., Antranikian, G., Maloney, S., Sharp, R. (1998) Thermophilic Degradation of Environmental Pollutants. Advances Biochem. Eng../Bio- technol. 61:155-169
• 2-09.002V
  Niehaus, F., Frey, B., Antranikian, G. (1997) Cloning and characterisation of a thermostable a-DNA polymerase from the hyperthermophilic archaeon Thermococcus sp. TY. GENE 204:153-158
• 2-09.003V
  Mutzel, A., Reinscheid, U. M., Antranikian, G., Müller, R. (1996) Isolation and characterization of thermophilic Bacillus strain, that degrades phenol and cresols as sole carbon source at 70 °C. Appl. Microbiol. Biotechnol. 46: 593-596
• 2-09.005V
  Canganella, F., Jones, W. J., Gambacorta, A., Antranikian, G. (1998) Thermococcus guaymasensis sp. nov. and Thermococcus aggregans sp. nov., two novel thermophilic archaea isolated from the Guaymas Basin hydrothermal vent site. Intern. Journal of System. Bacteriol. 48:1181-1185
• 2-09.007V
  Märkl, H. ; G. Reinhold: Biogas-Turmreaktor, ein neues Konzept in der anaeroben Abwasserreinigung; Chem. -Ing. Tech. (1994)
• 2-09.008V
  Friedmann, H. ; H. Märkl,"Der Einfluß von erhöhtem hydrostatischem Druck auf die Biogasproduktion, gwf Nr. 12 (1993) S. 689-698. ".
• 2-09.009V
  Friedmann, H. ; H. Märkl: Der Einfluß der Produktgase auf die mikrobiologische Methanbildung; gwf (1994)
• 2-09.010V
  Meyer-Jens, Th. ; Märkl, H. ; Schröder, A. ; Kesners, P. ; Matz, G: Application of a membrane probe for the analysis of mixtures of organic substances and dissolved gaseous components in anaerobic reactors by means of a mass spectrometer. Dechema, Biotechnology conferences (1992), Vol. 5, Part A, S. 391/393
• 2-09.011V
  Märkl, H.:Folien und Membranen als neue Elemente im Fermenterbau. forum mikrobiologie 12 (1989), S. 234/237
• 2-09.012V
  Märkl, H. , M. Lechner, F. Götz,"A New Dialysis Fermenter for the Production of High Concentrations of Extracellular Enzymes J. Ferment. Bioeng. Vol. 69 (1990), No. 4, S. 244/249. ".
• 2-09.013V
  Dubach, A. , H. Märkl,"Application of an Extended Kalman Filter Method for Monitoring High Density Cultivation of E. coli. J. Ferment. Bioeng. (1992), Vol. 73, No. 5, S. 296/402. ".
• 2-09.014V
  Ogbonna, J. Ch; H. Märkl,"Nutrient-split feeding strategy for dialysis cultivation of E. coli. Biotechnology and Bioengineering. (1993), 41, S. 1092/1100. ".
• 2-09.015V
  Märkl, H. , C. Zenneck, A. Ch. Dubach, J. Ch. Ogbonna: Cultivation of E. coli to high cell densities in a dialysis reactor. Appl. Microbiol. Biotechnol. (1993), 39, S. 48/52. ".
• 2-09.016V
  Rüdiger, A. , J. Ch. Ogbonna, H. Märkl, G. Antranikian: Effect of gassing, agitation, substrate supplementation and dialysis on the growth of an extremely thermophilic archaeon Pyrococcus woesei. Appl. Microbiol. Biotechnol. (1992), 37, S. 501/504.
• 2-09.017V
  Wilderer, P. A. ; H. Märkl,"Innovative Reactor Design to Treat Hazardous Wastes. In,"Biotechnology Applications in Hazardous Waste Treatment, Engineering Found. Conf. , Florida, USA, United Engineering Trustees Inc. (1989) S. 214/259. ".
• 2-09.018V
  Märkl, H. ; C. Zenneck; P. A. Wilderer: Membranbioreaktoren für den mikrobiellen Abbau von BTX-Aromaten; Wasser-Abwasser 132 (1991) Nr. 7. ".
• 2-09.019V
  Zenneck, C. ; H. Märkl,"Membrane-photo-reactor for microbial degradation of volatile and toxic substanced in,"Arendt, F. ; Annokkee, G. J. ; Bosmann, R. ; van den Brink, W. J. ,"Altlastensanierung '93, 1505-1506, Kluwer Academic Publishers, 1993. ".
• 2-09.020V
  Kurosawa H. , H. Märkl, C. Niebuhr-Redder; M. Matsumura,"Dialysis Bioreactor with Radial-Flow Fixed Bed for Animal Cell Culture, J. Ferment. Bioeng. , Vol. 72 (1991); No. 1, S. 41/45. ".
• 2-09.021V
  Bohmann. A. ; R. Pörtner; J. Schmieding; V. Kasche; H. Märkl: Integrated membrane dialysis bioreactor with radial-flow fixed bed - a new approach for continuous cultivation of animal cells,"Cytotechnology, 9,"51-57, 1992".
• 2-09.022V
  Bohmann, A. ; R. Pörtner; H. Märkl,"Evaluation of optimal process parameters for the membrane dialysis bioreactor with integrated radial fixed bed; In,"Kaminogawa, S. ; Ametani, A. ; Hachimura, S. ; Animal Cell Technology,"Basic & Applied Aspects, Vol. 5, Kluwer Academic Publishers, 1993. ".
• 2-09.023V
  Lüdemann, I.; R. Pörtner and H. Märkl,"Effect of NH3 on the cell growth of a hybridoma cell line; Cytotechnology 14,"11-20 (1994) ".
• 2-09.024V
  Pörtner, R.; A. Bohmann; I. Lüdemann and H. Märkl,"Estimation of specific glucose uptake rates in cultures of hybridoma cells,"Journal of Biotechnology 34,"237-246 (1994)".
• 2-09.025V
  Ong, C.P.; R. Pörtner; H. Märkl; Y. Yamazaki; K. Yasuda and M. Matsumura,"High density cultivation of hybridoma in charged porous carriers; Journal of Biotechnology 34,"259-268 (1994)".
• 2-09.026V
  Zumbusch, P.v., T. Meyer-Jens, G. Brunner and H. Märkl,"On-line monitoring of organic substances with high-pressure liquid chromatography (HPLC) during anaerobic fermentation of waste-water. Appl. Microbiol. Biotechnol. 42,"140-146 (1994)".
• 2-09.027V
  Märkl H. und R. Stegmann (Herausgeber),"Anaerobe Behandlung von festen und flüssigen Rückständen. Dechema-Monographie Band 130 (1994)".
• 2-09.028V
  Meyer-Jens, T.; G. Matz and H. Märkl,"On-line measurement of dissolved and gaseous hydrogen sulphide in anaerobic biogas reactors. Appl. Microbiol. Biotechnol. 43,"341 - 345 (1994)".
• 2-09.029V
  A. Bohmann; R. Pörtner and H. Märkl,"Performance of a membrane dialysis bioreactor with radial-flow fixed bed for the cultivation of a hybridoma cell line; Appl. Microbiol. Biotechnol. 43,"772-780 (1995) ".
• 2-09.030V
  O. Holst , A. Manelius, M, Krahe, H. Märkl, N. Raven and R. Sharp,"Thermophiles and Fermentation Technology, submitted to "Comparative Biochemistry and Physiology"".
• 2-09.034V
  Rüdiger, A., Sunna, A., Antranikian, G.,"Natural polysaccharides degrading enzymes, 316-340. In,"Enzyme Catalysis in Organic Synthesis, Vol. I., K. Drauz and H. Waldmann (eds.) VCH, Weinheim, 1995.".
• 2-09.035V
  Leuschner, C., Antranikian, G.,"Heat-stable enzymes from extremely thermophilic and hyperthermophilic microorganisms. World journal of Microbiology and Biotechnology, 11, 95-114, 1995.".
• 2-09.036V
  Rüdiger, A., Jorgensen, P.L., Antranikian, G.,"Isolation and characterization of a heat-stable pullulanase from the hyperthermophilic archaeon Pyrococcus woesei after gene cloning and expression of its gene in Escherichia coli, Appl. Environ. Microbiol., 61, 567-575, 1995.".
• 2-09.037V
  Sunna, A., Stoffregen, T. and Antranikian, G.,"Xylan degrading enzymes from thermophilic bacteria, pp. 97-99. In Dechema Biotechnology Conferences, Vol. 5A, G. Kreysa and A. J. Driesel (eds.), VCH, Weinheim, Germany, 1992.".
• 2-09.038V
  Antranikian, G., Rüdiger, A., Canganella, F., Klingeberg, M. and Sunna, A.,"Biodegradation of polymers at temperatures up to 130°C. J. M. S. Pure Appl. Chem. A32,"661-669, 1995.".
• 2-09.039V
  Sunna, A., Puls, J. and Antranikian, G.,"Characterization of the xylanolytic enzyme system of the extreme thermophilic anaerobic bacteria Thermotoga maritima, T. neapolitana and T. thermarum. Comp. Biochem. Physiol. (im Druck). ".
• 2-09.040V
  Canganella, F., Andrade, C.M., Antranikian, G.,"Characterization of amylolytic and pullulytic enzymes from thermophilic archaea and from a new Fervidobacterium species.Appl. Microbiol. Biotechnol., 42, 239-245, 1994.".
• 2-09.041V
  Andrade, C.M., Antranikian, G.,"Screening and investigation for thermostable xylanases in thermophilic and hyperthermophilic archaea. 17th European Congress on Biotechnology, 19.-23. Februar, Nice, France, 1995.".
• 2-09.042V
  Prowe, S.G., Antranikian, G.,"Thermoalkalibacter bogoriae, a new anaerobic thermoalkaliphile isolated from the east african Lake Bogoria.VAAM-Tagung 26.-29.03.1995, Stuttgart.".
• 2-09.043V
  Friedrich, A.B., Böckle, B., Müller, R., Antranikian, G.,"Keratin degradation by Fervidobacterium pennavorans and Streptomyces pactum. Biospektrum, Sonderausgabe, 32, 1995.".
• 2-09.044V
  Friedrich, A.B., Hippe, H., Antranikian, G.,"Characterization of the keratinolytic enzyme system from a new thermophilic anaerobic bacterium, Fervidobacterium pennavorans. Appl. Environ. Microbiol., eingereicht.".
• 2-09.045V
  Klingeberg, M., Galunsky, B., Sjoholm, C., Kasche, V., Antranikian, G.,"Purification and properties of a highly thermostable, sodium dodecyl sulfate-resistant and stereospecific proteinase from the extremely thermophilic archaeon Thermococcus stetteri. Appl. Environ. Microbiol, 61, 3098-3104, 1995.
• 2-09.046V
  Krahe, M., G. Antranikian and H. Märkl: Fermentation of extremophilic microorganisms. FEMS Microbiology Reviews 18 (1996) 271 - 285
• 2-09.047V
  Pörtner, R.; A. Schilling; I, Lüdemann and H. Märkl: High density fed-batch cultures for hybridoma cells performed with the aid of a kinetic model. Bioprocess Engineering 15 (1996) 117-124
• 2-09.048V
  Lüdemann, I., R. Pörtner, C. Schaefer, K. Schick, K. Srámková, K. Reher, M. Neumaier, F. Franék and H. Märkl: Improvement of the culture stability of non-anchorage-dependent animal cells grown in serum-free media through immobilization. Cytotechnology 19 (1996) 111-124
• 2-09.049V
  Reinhold, G., S. Merrath, F. Lennemann and H. Märkl: Modelling the hydrodynamics and the fluid mixing behaviour of a Biogas Tower Reactor. Chem. Eng. Sci. 51,17 (1996) 4065-4073
• 2-09.050V
  Pörtner, R., I. Lüdemann und H. Märkl: Dialysekulturen mit immobilisierten tierischen Zellen. Biospektrum 6 (1996) 47-49
• 2-09.051V
  Pörtner, R.; I. Lüdemann and H. Märkl: Dialysis cultures with immobilized hybridoma cells for effective production of monoclonal antibodies. Cytotechnology 23: 39-45 (1997)
• 2-09.052V
  Reinhold, G. and H. Märkl: Model based scale up and performance of the Biogas Tower Reactor for anaerobic waste water treatment. Wat. Res. 31, 8: 2057-2065 (1997)
• 2-09.053V
  Nakano, N.; M. Rischke, S. Sato and H. Märkl: Influence of acetic acid on the growth of Escherichia coli K12 during high-cell-density cultivation in a dialysis reactor. Appl. Microbiol. Biotechnol. 48: 597-601 (1997)
• 2-09.054V
  Becker, P.; I. Abu-Reesh; S. Markossian; G. Antranikian and H. Märkl: Determination of the kinetic parameters during continuous cultivation of the lipase-producing thermophile Bacillus sp. IHI-91 on olive oil. Appl. Microbiol. Biotechnol. 48:184-190 (1997)
• 2-09.055V
  Pörtner, R.; I. Lüdemann; K. Reher; M. Neumaier; H. Märkl:(1998) Fixed-bed dialysis culture of a transfectoma cell line producing chimeric Fab-fragments with "nutrient-split"-feeding strategy. Biotechnol. Tech. 12,7: 501-505 (1998)
• 2-09.056V
  Schwabe, J.O.; R. Pörtner and H. Märkl: Improving an on-line feeding strategy for fed-batch cultures of hybridoma cells by dialysis and "nutrient-split"-feeding. Bioproc. Eng. 20, 6: 475 - 484 (1999)
• 2-09.057V
  Pörtner, R. and Th. Schäfer: Modelling hybridoma cell growth and metabolism - A comparision of selected models and data; Journal of Biotechnology 49: 119-135 (1996)
• 2-09.058V
  Pörtner, R. and H. Märkl: Dialysis cultures. Appl. Microbiol. Biotechnol. 50: 403-414 (1998)
• 2-09.059V
  Fassnacht, D.; S. Rössing; N. Ghaussy and R. Pörtner: Influence of non-essential amino acids on apoptotic and necrotic death of mouse hybridoma cells in batch cultures. Biotechnology Letters 19: 35-38 (1997)
• 2-09.060V
  Pörtner, R.; S. Rössing; M. Koop and I. Lüdemann: Kinetic studies on hybridoma cells immobilized in fixed bed reactos. Biotechnology and Bioengineering, 55, 3: 535-541 (1997)
• 2-09.061V
  Pörtner, R.; S. Rössing und D. Fassnacht: Festbettreaktoren mit immobilisierten tierischen und menschlichen Zellen. Bioscope 9: 25-33 (1997)
• 2-09.062V
  Pörtner, R. and M. Koop: A model for oxygen supply in fixed bed reactors with immobilized hybridoma cells. Bioprocess Engineering 17: 269-275 (1997)
• 2-09.063V
  Fassnacht, D.; S. Rössing; J. Stange and R. Pörtner: Long-term cultivation of immortalised mouse hepatocytes in a high cell density fixed bed reactor. Biotechnology Techniques 12: 25-30 (1998)
• 2-09.064V
  Fassnacht, D.; S. Rössing; F. Franék; M. Al-Rubeai and R. Pörtner: Effect of BCL-2 Expression on hybridoma cell growth in serum-supplemented, protein-free and diluted media. Cytotechnology 26 (3): 219-225 (1998)
• 2-09.065V
  Fassnacht, D.; S. Rössing; R. Singh; M. Al-Rubeai and R. Pörtner: Influence of BCL-2 Expression on antibody productivity in high cell density hybridoma culture systems. Cytotechnology 30: 95-105 (1999)
• 2-09.066V
  Fassnacht, D. and R. Pörtner: Experimental and theoretical considerations on oxygen supply for animal cell growth in fixed bed reactors. Journal of Biotechnology 72 (3): 169 - 184 (1999)
• 2-09.067V
  Manusadzianas, L.; R. Vitkas; R. Pörtner and H. Märkl: Phytotoxicities of selected chemicals and industrial effluents to Nitellopsis obtusa cells, assessed by using a rapid electrophysiological Charophyte test. ATLA, 27: 379-386 (1999)
• 2-09.068V
  Nehring, D., P. Adamietz, N. M. Meenen and R. Pörtner: Perfusion cultures and modelling of oxygen uptake with three-dimensional chondrocyte pellets. Biotechnology Techniques 13: 701-706 (1999)
• 2-09.069V
  Pörtner, R.; D. Fassnacht and H. Märkl: Immobilization of mammalian cells in fixed bed reactors. BIOforum International 3: 140-141 (1999)
• 2-09.070V
  Fussenegger, M.; D. Fassnacht; R. Schwartz; J. A. Zanghi; M. Graf, J. E. Bailey and R. Pörtner: Regulated overexpression of the survival factor bcl-2 in CHO cells increases viable cell density in batch culture and decreases DNA release in extended fixed-bed cultivation. Cytotechnology 32: 45-61 (2000)
• 2-09.071V
  Märkl, H., Pietsch, T.: Mathematical Modelling and Kinetics of Anaerobic Digestion, erschienen in: Technik anaerober Prozesse. Dechema-Fachgespräche Umwelt-schutz (1998), S.69-82
• 2-09.072V
  Märkl, H.: Modeling of biogas reactors. In: H.-J. Rehm and G. Reed: Biotechnology, Wiley-VCH, Weinheim, Volume 11, pp. 529-560
• 2-09.073V
  Bertoldo C, Duffner F, Jørgensen PL, Antranikian G (1999) Pullulanase type I from Fervidobacterium pennavorans Ven5: cloning, sequencing, expression of the gene and biochemical characterization of the recombinant enzyme. Appl Environ Microbiol. 65, p 2084-2091
• 2-09.074V
  Duffner, F., Bertoldo, C., Andersen, Jens T., Wagner, K., and Antranikian G. (2000) A new thermoactive pullulanase from Desulfurococcus mucosus: cloning, sequencing, purification and characteriaztion of the recombinant enzyme after expression in Bacillus subtilis. J. Bacteriol. 182, 6331-6338
• 2-09.075V
  Bohlke K; Pisani FM; Vorgias CE; Frey B; Sobek H; Rossi M; Antranikian G (2000) PCR performance of the B-type DNA polymerase from the thermophilic euryarchaeonthermococcus aggregans improved by mutations in the Y-GG/A motif Nucleic Acids Res, 28, 3910-3917
• 2-09.076V
  Niehaus F; Peters A; Groudieva T; Antranikian G (2000) Cloning, expression and biochemical characterisation of a unique thermostable pullulan-hydrolysing enzyme from the hyperthermophilic archaeon Thermococcus aggregans FEMS Microbiol Lett 190:223-229
• 2-09.077V
  Grote, R., Li, L., Tamaoka, J., Kato, Chiaki, Horikoshi, K. and Antranikian, G. (1999) Thermococcus siculi sp. nov., a novel hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent at the Mid-Okinawa Trough. Extremophiles 3:55-62
• 2-09.078V
  Frillingos S, Linden A, Niehaus F, and Antranikian G. (2000) Cloning and expression of alpha-amylase from the hyperthermophilic archaeon Pyrococcus woesei in the moderately halophilic bacterium Halomonas elongata. J Appl Microbiol., 88, 495-503
• 2-09.079V
  Linden A, Niehaus F, Antranikian G. (2000) Single-step purification of a recombinant thermostable alpha-amylase after solubilization of the enzyme from insoluble aggregates. J Chromatogr B Biomed Sci Appl 737, 253-9
• 2-09.080V
  Niehaus F, Bertoldo C, Kahler M, Antranikian G. (1999) Extremophiles as a source of novel enzymes for industrial application. Appl Microbiol Biotechnol. 51: 711-729
• 2-09.081V
  Stefanova ME; Schwerdtfeger R; Antranikian G; Scandurra R. (1999) Heat-stable pullulanase from Bacillus acidopullulyticus: characterization and refolding after guanidinium chloride-induced unfolding. Extremophiles:147-52
• 2-09.082V
  Markossian, S.; P. Becker, H. Märkl and G. Antranikian: Isolation and characterization of lipid-degrading Bacillus thermoleovorans IHI-91 from an icelandic hot spring. Extremophiles 4: 365-371 (2000)
• 2-09.083V
  Becker, P. and H. Märkl: Modeling of olive oil degradation and oleic acid inhibition during chemostat and batch cultivation of Bacillus thermoleovorans IHI-91. Biotechnology and Bioengineering, 70,6: 632-637 (2000)
• 2-09.084V
  Feitkenhauer, H.; S. Schnicke, R. Müller and H. Märkl: Determination of kinetic parameters of the pheol-degrading thermophile Bacillus thermoleovorans sp. A2. Applied Microbiology and Biotechnology 57: 744-750 (2001)
• 2-09.085V
  Reimann, I. und H. Märkl (2001): Thermophile Mikroorganismen für die Reinigung fetthaltiger Abwässer. Sonderband Biokatalyse der DBU
• 2-09.086V
  Feitkenhauer, H.; S. Schnicke, R. Müller and H. Märkl (2001): Determination of kinetic parameters of the phenol-degrading thermophile Bacillus thermoleovorans sp. A2. Applied Microbiology and Biotechnology 57: 744-750
• 2-09.087V
  Fuchs, C.; D. Köster, S. Wiebusch, K. Mahr, G. Eisbrenner, H. Märkl (2002): Scale up of the dialysis fermentation for high cell density fermentation of E. coli. Journal of Biotechnology 93, 3, 243-251
• 2-09.088V
  Biller, F. K.; I. Kato, H. Märkl (2002): Effect of glucose, maltose, soluble starch, and CO2 on the growth of the hyperthermophilic archaeon Pyrococcus furiosus. Extremophiles 6: 161-166
• 2-09.089V
  Reimann, I.; A. Klatt, H. Märkl (2002): Behandlung fetthaltiger Abwässer der Lebensmittelindustrie mit einem thermophilen Mikroorganismus, Chemie Ingenieur Technik 74: 508-512
• 2-09.090V
  Pietsch, T.; R. Mehrwald, R. Grajetzki, J. Sens, T. Pakendorf, R. Ulrich, J. Kumpart, G. Matz, H. Märkl (2002): Macro- and microscopic in-situ observation of gas bubbles and sludge particles in a biogas tower reactor. Water Research 36: 2836-2842
• 2-09.091V
  Pietsch, T.; Mehrwald, R.; Grajetzki, R.; Sens, J.; Märkl, H. (2003):Sedimentation behaviour of sludge particles in a biogas tower reactor and the function of a hydrostatically pressurized sedimenter. Water Research 37 (5):1071-1079.
• 2-09.092V
  Fassnacht, D.; I. Reimann, R. Pörtner und H. Märkl: Scale-up von Festbettreak-toren zur Kultivierung tierischer Zellen. Chemie Ingenieur Technik 73: 1075 - 1079 (2001)
• 2-09.093V
  Frahm, B.; H.-C. Blank; P. Cornand; W. Oelßner; U. Guth; P. Lane; A. Munack; K. Johannsen and R. Pörtner: Determination of dissolved CO2 concentration and CO2 production rate of mammalian cell suspension culture based on off-gas measurement. Journal of Biotechnology 99/2: 133-148 (2002)
• 2-09.094V
  Frahm, B.; P. Lane; H. Atzert; A. Munack; M. Hoffmann; V. Hass and R. Pörtner: Adaptive, model-based control by the Open-Loop-Feedback-Optimal (OLFO) controller for the effective fed-batch cultivation of hybridoma cells. Biotechnology Progress 18: 1095-1103 (2002)
• 2-09.095V
  Nagel-Heyer, St. and R. Pörtner: Bioreactor cultivation for three-dimensional cartilage implants. G.I.T. Laboratory Journal 2: 66-67 (2002)
• 2-09.096V
  Frahm, B. und R. Pörtner: Reaktor-Abgasmessungen bei Zellkulturen, BIOforum 4, 2002
• 2-09.097V
  Reimann, I.; A. Klatt, H. Märkl (2002): Treatment of Wastewater Containing Fat from Food Industry with a Thermophilic Microorganism. Engineering in Life Sciences 2, 12:398-401.
• 2-09.098V
  Nehring, D.; P. Czermak; S. Gokorsch und R. Pörtner: Produktion retroviraler Partikel im Festbettreaktor. BioWorld 4: 14-15 (2003)
• 2-09.099V
  Frahm, B., Hass, V. C., Lane, P., Munack, A., Märkl, H. and R. Pörtner: Fed-batch Kultivierung tierischer Zellen - eine Herausforderung zur adaptiven, modellbasierten Steuerung, Chemie Ingenieur Technik, vol. 4, pp. 457 460 (2003)
• 2-09.100V
  Frahm, B., Lane, P., Märkl, H. and R. Pörtner: Improvement of a mammalian cell culture process by adaptive, model-based dialysis fed-batch cultivation and suppression of apoptosis, Bioprocess and Biosystems Engineering, vol. 26, pp. 1-10 (2003)
• 2-09.101V
  Krüger, M., Reimann, I., Märkl, H., Taube, J., Eckert, W. (2003): Aerobe thermophile Reinigung fetthaltiger Abwässer der Lebensmittelindustrie mit Bacillus thermoleovorans, Trankscript Sonderband 2003, 20-23
• 2-09.102V
  Brodersen, J., Rießen, S., Antranikian, G., Märkl, H. (2003): Umweltfreundliche Aufbereitung von Hühnerfedern mittels extremthermophiler Bakterien und thermostabilen Enzymen, Trankscript Sonderband 2003, 17-19
• 2-09.103V
  Nehring, D.; R. Gonzales; P. Czermak and R. Pörtner: Mathematical model of a mem-brane filtration process using ceramic membranes to increase retroviral pseudotype vector titer. Journal of Membrane Science 237: 25-38 (2004)
• 2-09.104V
  Pörtner, R.; J.-O. Schwabe and B. Frahm: Evaluation of selected control strategies for fed-batch cultures of a hybridoma cell line, Biotechnology and Applied Biochemistry 40: 47-55 (2004)
• 2-09.105V
  Nagel-Heyer, S.; R. Janßen; C. Goepfert; P. Adamietz; N.M. Meenen and R. Pörtner: Carti-lage cultivation on a calcium phosphate ceramic in a bioreactor system. Key Engineering Materials 264-268: 2119-2122 (2004)
• 2-09.106V
  Janssen, R.; S. Nagel-Heyer; Ch. Goepfert; R. Pörtner; D. Toykan; O. Krummhauer; M.M. Morlock; P. Adamietz; N.M. Meenen; W.M. Kriven; D.-K. Kim; A. Tampieri and G. Celotti: Cal-cium phosphate ceramics as substrate for cartilage cultivation. Ceramic Engineering and Sci-ence Proceedings 25, 4: 523-528 (2004)
• 2-09.107V
  Pörtner, R.; M. Seemuk; R.-Ch. Schlothauer; D. Elsser: Anaerobe Kultivierung von Lactococcus lactis im Festbettreaktor. Chemie-Ingenieur-Technik, 76, 10: 1599-1602 (2004)
• 2-09.108V
  Nagel-Heyer, S.; Ch. Goepfert; M.M. Morlock and R. Pörtner: Relationship between gross morphological and biochemical data of tissue engineered cartilage-carrier-constructs. Biotechnology Letters, 27(3): 187-192 (2005)
• 2-09.109V
  Kasche, V.; Z. Ignatova; H. Markl; W. Plate; N. Punckt; D. Schmidt; K. Wiegandt and B. Ernst: Ca2+ is a cofactor required for membrane transport and maturation and is a yield determining factor in high cell density penicillin amidase production. Biotechnol Prog 21(2):432-8 (2005)
• 2-09.110V
  Nagel-Heyer, S.; Ch. Goepfert; M.M. Morlock and R. Pörtner: Relationship between gross morphological and biochemical data of tissue engineered cartilage-carrier-constructs. Biotechnology Letters 27(3): 187-192 (2005)
• 2-09.111V
  Frahm, B.; P. Lane, A. Munack und R. Pörtner: Optimierung und Steuerung von Zellkultur-Fed-Batch-Prozessen mittels einer Kollokationsmethode. Chemie-Ingenieur-Technik, 77 (4), 429 – 435 (2005)
• 2-09.112V
  Nagel-Heyer, S.; Ch. Goepfert; P. Adamietz; N.M. Meenen; J.-P. Petersen and R. Pörtner: Flow-chamber bioreactor culture for generation of three-dimensional cartilage-carrier-constructs. Bioprocess and Biosystems Engineering, 27: 273-280 (2005)
• 2-09.113V
  Meenen, N.M., P. Ueblacker, R. Pörtner, C. Göpfert, S. Nagel-Heyer, J. P. Petersen und P. Adamietz: ; „ Knorpel aus dem Labor-eine Sackgasse?“,. Arthroskopie 18: 245-252 (2005)
• 2-09.114V
  Pörtner, R.; St. Nagel-Heyer; Ch. Goepfert; P. Adamietz and N. M. Meenen: Bioreactor design for tissue engineering. Journal of Bioengineering and Bioscience, 100(3): 235-245 (2005)

Dissertationen

• 2-09.001D
  Reinhold, Georg: Maßstabsvergrößerung eines Biogas-Turmreaktors zur anaeroben Abwasserreinigung, 1996
• 2-09.002D
  Lüdemann, Ines: "Nutrient-Split"-Fütterungsstrategie für suspendierte und immobilisierte nicht-adhärente tierische Zellen, 1997
• 2-09.003D
  Krahe, Martin: Kultivierung von Mikroorganismen zu hohen Zelldichten, 1997.
• 2-09.004D
  Müller, Birgit: Untersuchungen zum Abbau polyzyklischer aromatischer Kohlenwasserstoffe (PAK) durch thermophile Mikroorganismen, 1998.
• 2-09.005D
  Polomski, Andreas: Einfluß des Stofftransports auf die Methanbildung in Biogas-Reaktoren, 1998
• 2-09.006D
  Feitkenhauer, Heiko: Biodegradation of aliphatic and aromatic hydrocarbons at high temperatures, 1998
• 2-09.007D
  Pietsch, Torsten: Rückhalt suspendierter Biomasse und örtliche Verteilung gelöster Gase in einem Biogas-Turmreaktor, 2000
• 2-09.008D
  Becker, Peter: Der biologische Abbau von Fetten durch aerobe, thermophile Bakterien: Untersuchungen zur Kinetik und zum Einsatz in der Abwassertechnik, 1999
• 2-09.009D
  Fassnacht, Dieter: Fixed-bed reactors for the cultivation of animal cells, 2000
• 2-09.010D
  Schwabe, Jan-Oliver: Feeding Strategies for Fed-Batch Cultures of Animal Cells, 2000
• 2-09.011D
  Niehaus, F.: Klonierung und Charakterisierung einer neuen, thermostabilen a-DNA Polymerase, 1996
• 2-09.012D
  Prowe, S.: Characterization of Extracellular Amylolytic Enzymes and Sodium Coupled Energy Transduction of a Newly Isolated Thermoalkaliphilic Bacterium, Thermoalcalibacter bogoriae, 1996
• 2-09.013D
  Schwerdtfeger, R.: Structure/Function Relationships of Pullulanase and Glutamate Dehydrogenase from the Hyperthermophilic Archaeon Pyrococcus woesei, 1997
• 2-09.014D
  Hebenbrock, S.: Isolierung und Charakterisierung von thermophilen Mikroorganismen und deren Untersuchungen zum mikrobiellen Abbau von Naphthalin und Benzoesäure, 1998
• 2-09.015D
  Reinigung und biochemische Charakterisierung einer thermostabilena-Amylase aus Pyrococcus woesei nach Klonierung und Expression inEscherichia coli und Halomonas elongata. 1999
• 2-09.016D
  Kähler M. Cloning and characterization of DNA polymerase from the hyperthermophilic Crenoarchaeon Pyrobaculum islandicum. 1999
• 2-09.017D
  Serour E., Production, Purification and characterization of novel thermoactive glucoamylases from hyperacidophilic archaea Thermoplasma acidophilum, Picrophilus torridus and Picrophilus oshimae. 2000
• 2-09.018D
  Fuchs, C.: Einsatz einer Dialysemembran bei der Fermentation von E. coli. 2002
• 2-09.019D
  Köster, D.: Proteinexpression in dichten Kulturen von E. coli. 2002
• 2-09.020D
  Biller, K.: Fermentation hyperthermophiler Mikroorganismen. 2002
• 2-09.021D
  Grote R., Thermococcus siculi sp. nov., a novel hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent at the Mid-Okinawa Trough. Extremophiles 3:55-62. (1999)
• 2-09.022D
  Hansen, G.: Der Venturi-Bioreaktor: Ein System mit hoher Stoffaustauschleistung zwischen Gas- und Flüssigphase. 2001
• 2-09.023D
  Reimann, I.: Thermophile Behandlungsverfahren zur biologischen Reinigung fetthaltiger Abwässer. 2003
• 2-09.024D
  Frahm, B.: Adaptive, modellgestützte Prozessführung von Suspensionskulturen tierischer Zellen. 2003
• 2-09.025D
  Nagel-Heyer, St.: Ingenieurtechnische Aspekte bei der Herstellung von dreidimensionalen Knorpel-Träger-Konstrukten. 2004
• 2-09.026D
  Brodersen, J.: Biotechnologische Verfahren zum Abbau von Federn
• 2-09.027D
  Göpel, Ph.: Pektinolytische Enzyme aus thermophilen Bakterien: Wirkungsweise auf Bastfasern, Charakterisierung und Produktion

Habilitationen

• 2-09.001H
  Pörtner, R.: Reaktionstechnik der Kultur tierischer Zellen. 1997